Evaluation of Advanced Glycation End Products, Oxidative Stress and Antioxidants and their Relationship in Diabetic patients

Abstract

Objective: Diabetes Mellitus (DM) is a major public health problem in developed countries; which also plays a vital role in providing information about advanced glycation end products (AGEs) and these induce oxidative stress (OS). The aim of this study is to evaluate the AGEs, OS, antioxidant, and lipid profile parameters in sera of DM patients. Methods: The current study includes 82 subjects (50 patients with type II DM and 32 healthy subjects, men and women). Demographic factors and medication intake of every subject were obtained. The advanced glycation end products (AGEs), oxidative stress (OS), antioxidant, and lipid profile parameters were measured using a spectrometric method and ELISA test. Results: The present study elucidated that the serum levels of CML, CEL, Pyralline, 8-OHdG, and MDA were significantly increased in diabetic patients compared with the healthy group. The serum levels of GSH, NO, and GSH-Px were significantly decreased in patients with DM rather than in the healthy group. While the serum levels of CAT and SOD were non-significantly decreased in patients with DM. Conclusion: Meanwhile, the elevation of AGEs and OS are associated with DM patients. NO and CML could be potential biomarkers for DM.

Keywords

• Advanced Glycation End products (AGEs), Oxidative Stress (OS), Antioxidants, lipid profile, Diabetes Mellitus (DM)