In this feasibility study, high-resolution neutron tomography is used to investigate the water distribution in polymer electrolyte membrane fuel cells (PEMFCs). Two PEMFCs were built up with two different gas diffusion layers (GDLs) namely Sigracet® SGL-25BC and Freudenberg H14C10, respectively. High-resolution neutron tomography has the ability to display the water distribution in the flow field channels and the GDLs, with very high accuracy. Here, we found that the water distribution in the cell equipped with the Freudenberg H14C10 material was much more homogenous compared to the cell with the SGL-25BC material.