Effect of Biosynthesized ZnO Nanoparticles on Multi-Drug Resistant Pseudomonas Aeruginosa
Syed Ghazanfar Ali,
Mohammad Azam Ansari,
Mohammad A. Alzohairy,
Mohammad N. Alomary,
Mohammad Jalal,
Sami AlYahya,
Sarah Mousa Maadi Asiri,
Haris M. Khan
Affiliations
Syed Ghazanfar Ali
Department of Microbiology, Nanotechnology and Antimicrobial Drug Resistance Research Laboratory, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India
Mohammad Azam Ansari
Department of Epidemic Disease Research, Institute for Research & Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia
Mohammad A. Alzohairy
Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Qassim 51431, Saudi Arabia
Mohammad N. Alomary
National Center for Biotechnology, Life Science and Environmental Research Institute, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11442, Saudi Arabia
Mohammad Jalal
Department of Microbiology, Nanotechnology and Antimicrobial Drug Resistance Research Laboratory, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India
Sami AlYahya
National Center for Biotechnology, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11442, Saudi Arabia
Sarah Mousa Maadi Asiri
Department of Biophysics, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia
Haris M. Khan
Department of Microbiology, Nanotechnology and Antimicrobial Drug Resistance Research Laboratory, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India
Synthesis of nanoparticles using the plants has several advantages over other methods due to the environmentally friendly nature of plants. Besides being environmentally friendly, the synthesis of nanoparticles using plants or parts of the plants is also cost effective. The present study focuses on the biosynthesis of zinc oxide nanoparticles (ZnO NPs) using the seed extract of Butea monsoperma and their effect on to the quorum-mediated virulence factors of multidrug-resistant clinical isolates of Pseudomonas aeruginosa at sub minimum inhibitory concentration (MIC). The synthesized ZnO NPs were characterized by different techniques, such as Fourier transform infra-red spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), and transmission electron microscopy (TEM). The average size of the nanoparticles was 25 nm as analyzed by TEM. ZnO NPs at sub MIC decreased the production of virulence factors such as pyocyanin, protease and hemolysin for P. aeruginosa (p ≤ 0.05). The interaction of NPs with the P. aeruginosa cells on increasing concentration of NPs at sub MIC levels showed greater accumulation of nanoparticles inside the cells as analyzed by TEM.
