The Expression Analysis of Nanog in the Developing Rat Myocardial Tissues

Abstract

Objectives: To investigate the expression dynamic of nanog gene in the development of rat myocardial tissues. Methods: SD rats were studied at 5 time points before and after birth. The techniques of immunohistochemistry, immunofluorescence, western blotting and RT-PCR were used to investigate the expression of nanog gene in the rat myocardial tissues at different embryonic (E) and postnatal (P) stages, and image analysis system was used for the quantitative analysis. Results: The immunohistochemistry, immunofluorescence and western blotting analyses have shown that expression of nanog protein was highest in the rat myocardial tissues at E18, then it gradually declined at postnatal stages (PConclusion: The mRNA transcription and protein translation of nanog gene in the rat heart gradually decreased with every consecutive growth stage. This indicates that nanog gene has potential regulatory functions in the differentiation of myocardial cells during rat development.

Keywords

• Nanog
• Immunohistochemistry
• Western blotting
• RT-PCR
• Myocardial tissue
• Rat heart