ROC-Analysis of the Results as regards <I>B. pseudomallei</I> and <I>B. mallei</I> Agents Detection Using Solid-Phase ELISA

Abstract

Analyzed are operational characteristic curves (ROC-curves) of solid-phase ELISA variants with immune sera to cellular and extracellular Burkholderia antigens with the aim to determine the most effective ones for B. pseudomallei and B. mallei antigen detection. It is shown that among solid-phase ELISA variants with immune sera to cellular and extracellular antigens, those with sera to homologous antigens are the most effective. Demonstrated is the possibility of application of solid-phase ELISA variants with immune sera to antigens of B. thailandensis and B. cepacia strains for identification and typing of glanders and melioidosis agents. Assessed are prospects of extracellular antigen application for differentiation between closely related species of “ pseudomallei ” group.

Keywords

• roc-анализ
• буркхольдерии
• твердофазный иммуноферментный анализ
• клеточные и экстрацеллюлярные антигены
• сыворотка
• burkholderia pseudomallei
• b mallei
• roc-analysis
• burkholderia
• enzyme-linked immunosorbent assay
• cellular and extracellular antigens
• serum