Superparamagnetic Iron Oxide Nanoparticles Decorated Mesoporous Silica Nanosystem for Combined Antibiofilm Therapy
Elena Álvarez,
Manuel Estévez,
Alvaro Gallo-Cordova,
Blanca González,
Rafael R. Castillo,
María del Puerto Morales,
Montserrat Colilla,
Isabel Izquierdo-Barba,
María Vallet-Regí
Affiliations
Elena Álvarez
Departamento de Química en Ciencias Farmacéuticas, Faculdad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain
Manuel Estévez
Departamento de Química en Ciencias Farmacéuticas, Faculdad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain
Alvaro Gallo-Cordova
Instituto de Ciencia de Materiales de Madrid, ICMM/CSIC, Sor Juana Inés de la Cruz 3, 28049 Madrid, Spain
Blanca González
Departamento de Química en Ciencias Farmacéuticas, Faculdad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain
Rafael R. Castillo
Departamento de Química en Ciencias Farmacéuticas, Faculdad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain
María del Puerto Morales
Instituto de Ciencia de Materiales de Madrid, ICMM/CSIC, Sor Juana Inés de la Cruz 3, 28049 Madrid, Spain
Montserrat Colilla
Departamento de Química en Ciencias Farmacéuticas, Faculdad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain
Isabel Izquierdo-Barba
Departamento de Química en Ciencias Farmacéuticas, Faculdad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain
María Vallet-Regí
Departamento de Química en Ciencias Farmacéuticas, Faculdad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, 28040 Madrid, Spain
A crucial challenge to face in the treatment of biofilm-associated infection is the ability of bacteria to develop resistance to traditional antimicrobial therapies based on the administration of antibiotics alone. This study aims to apply magnetic hyperthermia together with controlled antibiotic delivery from a unique magnetic-responsive nanocarrier for a combination therapy against biofilm. The design of the nanosystem is based on antibiotic-loaded mesoporous silica nanoparticles (MSNs) externally functionalized with a thermo-responsive polymer capping layer, and decorated in the outermost surface with superparamagnetic iron oxide nanoparticles (SPIONs). The SPIONs are able to generate heat upon application of an alternating magnetic field (AMF), reaching the temperature needed to induce a change in the polymer conformation from linear to globular, therefore triggering pore uncapping and the antibiotic cargo release. The microbiological assays indicated that exposure of E. coli biofilms to 200 µg/mL of the nanosystem and the application of an AMF (202 kHz, 30 mT) decreased the number of viable bacteria by 4 log10 units compared with the control. The results of the present study show that combined hyperthermia and antibiotic treatment is a promising approach for the effective management of biofilm-associated infections.