Nature Communications (May 2020)

Inter-domain dynamics in the chaperone SurA and multi-site binding to its outer membrane protein clients

  • Antonio N. Calabrese,
  • Bob Schiffrin,
  • Matthew Watson,
  • Theodoros K. Karamanos,
  • Martin Walko,
  • Julia R. Humes,
  • Jim E. Horne,
  • Paul White,
  • Andrew J. Wilson,
  • Antreas C. Kalli,
  • Roman Tuma,
  • Alison E. Ashcroft,
  • David J. Brockwell,
  • Sheena E. Radford

DOI
https://doi.org/10.1038/s41467-020-15702-1
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 16

Abstract

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The chaperone SurA is involved in outer membrane protein (OMP) biogenesis in Gram-negative bacteria, but its mechanism of action is not fully understood. Combining mass spectrometric, biophysical and computational approaches, the authors here show how the conformational dynamics of SurA facilitate OMP binding.