Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection

Amanda Labora; Amanda Creech; Hailey Lee; Erin Tabornal; Caius Radu; Timothy Donahue

STAR Protocols (Dec 2023)

Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection

Amanda Labora,
Amanda Creech,
Hailey Lee,
Erin Tabornal,
Caius Radu,
Timothy Donahue

Affiliations

Amanda Labora: Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA; Corresponding author
Amanda Creech: Department of Molecular and Medical Pharmacology, Ahmanson Translational Imaging Division, University of California, Los Angeles, Los Angeles, CA 90095, USA
Hailey Lee: Department of Molecular and Medical Pharmacology, Ahmanson Translational Imaging Division, University of California, Los Angeles, Los Angeles, CA 90095, USA
Erin Tabornal: Department of Molecular and Medical Pharmacology, Ahmanson Translational Imaging Division, University of California, Los Angeles, Los Angeles, CA 90095, USA
Caius Radu: Department of Molecular and Medical Pharmacology, Ahmanson Translational Imaging Division, University of California, Los Angeles, Los Angeles, CA 90095, USA
Timothy Donahue: Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA; Corresponding author

Journal volume & issue: Vol. 4, no. 4
p. 102698

Abstract

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Summary: We report a technique to generate a murine model of lung metastases by selectively injecting tumor cells into the right heart ventricle under ultrasound guidance. First, we describe cell preparation and reference animal preparation as previously described. We then detail the technique using a previously described 3D-printed instrument stabilization device. Finally, we describe tumor growth surveillance using bioluminescent imaging.For complete details on the use and execution of this protocol, please refer to Labora et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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