Nature Communications (Nov 2022)

Spatially resolved proteomics via tissue expansion

  • Lu Li,
  • Cuiji Sun,
  • Yaoting Sun,
  • Zhen Dong,
  • Runxin Wu,
  • Xiaoting Sun,
  • Hanbin Zhang,
  • Wenhao Jiang,
  • Yan Zhou,
  • Xufeng Cen,
  • Shang Cai,
  • Hongguang Xia,
  • Yi Zhu,
  • Tiannan Guo,
  • Kiryl D. Piatkevich

DOI
https://doi.org/10.1038/s41467-022-34824-2
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 13

Abstract

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Spatially resolved proteomics is an emerging approach for mapping proteome heterogeneity. Here, the authors report a method based on the combination of hydrogel-based tissue transformation with mass spectrometry-based proteomics, that enables proteome profiling with a lateral resolution of 160 µm.