Journal of Islamic International Medical College (Sep 2019)
Effect of Betulinic Acid Vs Simvastatin on Hypelipidemic Mice Model
Abstract
Objective: To compare the effect of Betulinic acid and Simvastatin on Triglycerides and Low Density Lipoprotein Cholesterol (LDL-C) in Balb/C Mice. Study Design: Experimental randomized control trial. Place and Duration of Study: Study was conducted at Biochemistry department Islamic International Medical College Rawalpindi in collaboration with National Institute of Health Islamabad from November 2017 to April 2018. Materials and Methods: A total of 40 mice were randomly divided into 4 groups. Excluding one of the 4 groups as negative control, the remaining three were fed with high fat diet for 21 days to achieve raised levels of Triglycerides and Low density lipoprotein. After that out of the three high fat diet fed groups, one group was left untreated considering the positive control group and of the other 2 groups one was treated with simvastatin and 2nd one was given betulinic acid for the next 21 days. Sampling was done by cardiac puncture on 42 day. Triglycerides and LDL-C levels were measured in serum samples. Data thus obtained was analyzed by one way ANOVA through SPSS 21. Results: Study showed that positive control group showed a rise in mean triglycerides levels from 130mg/dL to 220mg/dL and mean LDL-C from 23mg/dL to 46mg/dL. Betulinic acid showed significantly better control than simvastatin as mean serum levels of Triglycerides were 146mg/dL as compared to 178mg/dL and mean serum levels of LDL-C were 28mg/dL as compared to 34mg/dL of simvastatin. Considering the p<0.05, TGs had a (p<0.001) and LDL-C had a (p<0.001). Conclusion: Betulinic acid showed a far better control over the levels of Triglycerides and LDL-C in HFD fed Balb/C mice as compared to simvastatin.
