Analysis on the Screening of an Endophytic Fungi from Artemisia Sacrorum Ledeb Against Wheat Scab and Mass Spectrographic Analysis

Abstract

Twelve endophytic fungus strain from artemisia sacrorum ledeb were isolated and purified with the method of surface disinfection and tip mycelium selection. The activity of eight strains of endophytic fermentation broth against wheat scab were studied by mycelial growth rate method. Most active strain were identified as Fusarium Redolens by the combination of morphology and ITS rDNA gene sequence. A mass spectrometry method was established to analyze the main metabolites. The results showed that the endophytic fungus (No: 20202707) from artemisia sacrorum ledeb demonstrated the strongest inhibitory effect on Fusarium graminearum, and the inhibitory rate was 65.27%. Its main metabolites were 3, 4-dihydrocoumarin, 5′deoxyribonuc leoside, Harmala alkaloid, benzofuran and benzothiazole.

Keywords

• artemisia sacrorum ledeb
• endophytic fungi
• biological activity
• secondary metabolites