Genetic diversity analysis of Cinnamomum cassia Presl and Cinnamomum cassia Presl var. macrophyllum Chu based on ISSR

Abstract

ISSR technology can provide detailed data on the genetic information of species. In this study, ISSR was used to find dominant bands at the DNA level to stably identify Cinnamomum cassia Presl and Cinnamomum cassia Presl var. macrophyllum Chu, and Cinnamomum cassia Presl from different production areas. The results showed that primer UBC834 had dominant bands for Cinnamomum cassia Presl and Cinnamomum cassia Presl var. macrophyllum Chu samples, and primer UBC876 had dominant bands for Cinnamomum cassia Presl samples from different production areas. By analyzing the data of ISSR, it was found that the clustering results and the results of principal component analysis were consistent with the results of electropherograms and were divided into five categories. At the same time, the polymorphism analysis showed that Cinnamomum cassia Presl germplasm resources were rich in genetic diversity. In addition, the results of genetic clustering analysis showed that when the GS was higher than 0.88, it was possible to distinguish Cinnamomum cassia Presl and Cinnamomum cassia Presl var. macrophyllum Chu. As the genetic similarity coefficient increased, Cinnamomum cassia Presl from different production areas could be distinguished. The results of this study provide a scientific basis for the development and utilization of the germplasm resources of Cinnamomum cassia Presl and Cinnamomum cassia Presl var. macrophyllum Chu, which can meet the economic and ecological needs. It also provides a new perspective for the conservation of biodiversity and biological evolution.

Keywords

• ISSR
• Molecular marker
• Cinnamomum cassia Presl
• Genetic diversity relatedness