Metal Oxide Nanostructures Enhanced Microfluidic Platform for Efficient and Sensitive Immunofluorescence Detection of Dengue Virus
Pareesa Pormrungruang,
Supranee Phanthanawiboon,
Sukittaya Jessadaluk,
Preeda Larpthavee,
Jiraphon Thaosing,
Adirek Rangkasikorn,
Navaphun Kayunkid,
Uraiwan Waiwijit,
Mati Horprathum,
Annop Klamchuen,
Tanapan Pruksamas,
Chunya Puttikhunt,
Takao Yasui,
Mitra Djamal,
Sakon Rahong,
Jiti Nukeaw
Affiliations
Pareesa Pormrungruang
College of Materials Innovation and Technology, King Mongkut’s Institute of Technology Ladkrabang, Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand
Supranee Phanthanawiboon
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Sukittaya Jessadaluk
College of Materials Innovation and Technology, King Mongkut’s Institute of Technology Ladkrabang, Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand
Preeda Larpthavee
College of Materials Innovation and Technology, King Mongkut’s Institute of Technology Ladkrabang, Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand
Jiraphon Thaosing
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Adirek Rangkasikorn
College of Materials Innovation and Technology, King Mongkut’s Institute of Technology Ladkrabang, Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand
Navaphun Kayunkid
College of Materials Innovation and Technology, King Mongkut’s Institute of Technology Ladkrabang, Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand
Uraiwan Waiwijit
National Electronics and Computer Technology Center, National Science and Development Agency, Pathumtani 12120, Thailand
Mati Horprathum
National Electronics and Computer Technology Center, National Science and Development Agency, Pathumtani 12120, Thailand
Annop Klamchuen
National Nanotechnology Center, National Science and Development Agency, Pathumtani 12120, Thailand
Tanapan Pruksamas
National Center for Genetic and Engineering and Biotechnology (BIOTEC), National Science and Development Agency, Pathumtani 12120, Thailand
Chunya Puttikhunt
National Center for Genetic and Engineering and Biotechnology (BIOTEC), National Science and Development Agency, Pathumtani 12120, Thailand
Takao Yasui
Department of Life Science and Technology, Tokyo Institute of Technology, B2-521, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan
Mitra Djamal
Department of Physics, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Bandung 46132, Indonesia
Sakon Rahong
College of Materials Innovation and Technology, King Mongkut’s Institute of Technology Ladkrabang, Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand
Jiti Nukeaw
College of Materials Innovation and Technology, King Mongkut’s Institute of Technology Ladkrabang, Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand
Rapid and sensitive detection of Dengue virus remains a critical challenge in global public health. This study presents the development and evaluation of a Zinc Oxide nanorod (ZnO NR)-surface-integrated microfluidic platform for the early detection of Dengue virus. Utilizing a seed-assisted hydrothermal synthesis method, high-purity ZnO NRs were synthesized, characterized by their hexagonal wurtzite structure and a high surface-to-volume ratio, offering abundant binding sites for bioconjugation. Further, a comparative analysis demonstrated that the ZnO NR substrate outperformed traditional bare glass substrates in functionalization efficiency with 4G2 monoclonal antibody (mAb). Subsequent optimization of the functionalization process identified 4% (3-Glycidyloxypropyl)trimethoxysilane (GPTMS) as the most effective surface modifier. The integration of this substrate within a herringbone-structured microfluidic platform resulted in a robust device for immunofluorescence detection of DENV-3. The limit of detection (LOD) for DENV-3 was observed to be as low as 3.1 × 10−4 ng/mL, highlighting the remarkable sensitivity of the ZnO NR-integrated microfluidic device. This study emphasizes the potential of ZnO NRs and the developed microfluidic platform for the early detection of DENV-3, with possible expansion to other biological targets, hence paving the way for enhanced public health responses and improved disease management strategies.
