Frontiers in Genetics (Mar 2021)

Immunogenomic Profiling Demonstrate AC003092.1 as an Immune-Related eRNA in Glioblastoma Multiforme

  • Xiao-Yu Guo,
  • Sheng Zhong,
  • Zhen-Ning Wang,
  • Tian Xie,
  • Hao Duan,
  • Jia-Yu Zhang,
  • Guan-Hua Zhang,
  • Lun Liang,
  • Run Cui,
  • Hong-Rong Hu,
  • Jie Lu,
  • Yi Wu,
  • Jia-Jun Dong,
  • Zhen-Qiang He,
  • Yong-Gao Mou

DOI
https://doi.org/10.3389/fgene.2021.633812
Journal volume & issue
Vol. 12

Abstract

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Enhancer RNAs, a type of long non-coding RNAs (lncRNAs), play a critical role in the occurrence and development of glioma. RNA-seq data from 161 glioblastoma multiforme (GBM) samples were acquired from The Cancer Genome Atlas database. Then, 70 eRNAs were identified as prognosis-related genes, which had significant relations with overall survival (log-rank test, p < 0.05). AC003092.1 was demonstrated as an immune-related eRNA by functional enrichment analysis. We divided samples into two groups based on AC003092.1 expression: AC003092.1 High (AC003092.1_H) and AC003092.1 Low (AC003092.1_L) and systematically analyzed the influence of AC003092.1 on the immune microenvironment by single-sample gene-set enrichment analysis and CIBERSORTx. We quantified AC003092.1 and TFPI2 levels in 11 high-grade gliomas, 5 low-grade gliomas, and 7 GBM cell lines. Our study indicates that AC003092.1 is related to glioma-immunosuppressive microenvironment, and these results offer innovative sights into GBM immune therapy.

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