Effect of intestinal fungal dysbiosis on corneal wound healing in mice

Lu-Lu Jia; Ding-Li Lu; Su-Su Liu; Yu-Qing Chen; Zhi-Jie Li; Li-Ya Wang

doi:10.3980/j.issn.1672-5123.2019.5.04

Guoji Yanke Zazhi (May 2019)

Effect of intestinal fungal dysbiosis on corneal wound healing in mice

Lu-Lu Jia,
Ding-Li Lu,
Su-Su Liu,
Yu-Qing Chen,
Zhi-Jie Li,
Li-Ya Wang

Affiliations

Lu-Lu Jia: People's Hospital of Zhengzhou University, Henan Eye Hospital, Henan Eye Institute, Henan Provincial People's Hospital, Henan Key Laboratory of Ophthalmology and Vision Science, Zhengzhou 450003, Henan Province, China
Ding-Li Lu: People's Hospital of Zhengzhou University, Henan Eye Hospital, Henan Eye Institute, Henan Provincial People's Hospital, Henan Key Laboratory of Ophthalmology and Vision Science, Zhengzhou 450003, Henan Province, China
Su-Su Liu: People's Hospital of Zhengzhou University, Henan Eye Hospital, Henan Eye Institute, Henan Provincial People's Hospital, Henan Key Laboratory of Ophthalmology and Vision Science, Zhengzhou 450003, Henan Province, China
Yu-Qing Chen: People's Hospital of Zhengzhou University, Henan Eye Hospital, Henan Eye Institute, Henan Provincial People's Hospital, Henan Key Laboratory of Ophthalmology and Vision Science, Zhengzhou 450003, Henan Province, China
Zhi-Jie Li: People's Hospital of Zhengzhou University, Henan Eye Hospital, Henan Eye Institute, Henan Provincial People's Hospital, Henan Key Laboratory of Ophthalmology and Vision Science, Zhengzhou 450003, Henan Province, China; Institute of Ophthalmology, Jinan University Medical School, Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, Guangdong Province, China
Li-Ya Wang: People's Hospital of Zhengzhou University, Henan Eye Hospital, Henan Eye Institute, Henan Provincial People's Hospital, Henan Key Laboratory of Ophthalmology and Vision Science, Zhengzhou 450003, Henan Province, China

DOI: https://doi.org/10.3980/j.issn.1672-5123.2019.5.04
Journal volume & issue: Vol. 19, no. 5
pp. 724 – 728

Abstract

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AIM: To investigate the effect of intestinal fungal dysbiosis induced by antifungal drug on corneal wound healing in mice.METHODS: Male C57BL/6J mice(free of eye disease)were divided into two groups randomly: control(Ctrl)group and amphotericin B treated(Amph)group, The Ctrl group was given a normal diet, and the Amph group was supplemented with amphotericin B to induce intestinal fungal dysbiosis. After 4wk intervention, corneal epithelial trauma was implemented in both groups. Corneal fluorescein staining was used to evaluate the corneal wound area dynamically. Immunofluorescence staining was applied to observe the changes of corneal epithelial cells and inflammatory cells. HE staining was used to assess the change of corneal thickness.RESULTS: Compared with Ctrl group, Amph group had delayed re-epithelialization rate and wound repair, less inflammatory cells and thinner corneal.CONCLUSION: Intestinal fungal dysbiosis delays the corneal wound healing, leading to a weak inflammatory response.

Published in Guoji Yanke Zazhi

ISSN: 1672-5123 (Print)
Publisher: Press of International Journal of Ophthalmology (IJO PRESS)
Country of publisher: China
LCC subjects: Medicine: Ophthalmology
Website: http://ies.ijo.cn/

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