Soybean hairy roots produced in vitro by Agrobacterium rhizogenes-mediated transformation
Li Chen,
Yupeng Cai,
Xiujie Liu,
Chen Guo,
Shi Sun,
Cunxiang Wu,
Bingjun Jiang,
Tianfu Han,
Wensheng Hou
Affiliations
Li Chen
National Center for Transgenic Research in Plants, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yupeng Cai
National Center for Transgenic Research in Plants, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Xiujie Liu
National Center for Transgenic Research in Plants, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Chen Guo
National Center for Transgenic Research in Plants, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Shi Sun
Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Cunxiang Wu
Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Bingjun Jiang
Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Tianfu Han
Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Wensheng Hou
National Center for Transgenic Research in Plants, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Soybean is one of the world's most important oil and protein crops. Efficient transformation is a key factor for the improvement of soybean by genetic modification. We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean and the induction of hairy root development in vitro. Cotyledons with 0.5-cm hypocotyls were cut from 5-day-old seedlings and used as explants. After infection and co-cultivation, hairy roots were produced in induction culture medium after 10–12 days. Using this method, 90%–99% of the infected explants of five different cultivars produced hairy roots within one month. Observations using reporter constructs showed that 30%–60% of the hairy roots induced were transformed. Based on high transformation efficiency and short transformation period, this method represents an efficient and rapid platform for study of soybean gene function.
