Cell Death and Disease (2021-05-01)

Circ_0001367 inhibits glioma proliferation, migration and invasion by sponging miR-431 and thus regulating NRXN3

  • Liang Liu,
  • Peng Zhang,
  • Xuchen Dong,
  • Haoran Li,
  • Suwen Li,
  • Shan Cheng,
  • Jiaqi Yuan,
  • Xuejun Yang,
  • Zhiyuan Qian,
  • Jun Dong

DOI
https://doi.org/10.1038/s41419-021-03834-1
Journal volume & issue
Vol. 12, no. 6
pp. 1 – 12

Abstract

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Abstract Many studies have reported that circular RNAs play a vital role in the malignant progression of human cancers. However, the role and underlying mechanism of circRNAs in the development of gliomas have not been fully clarified. In this study, we found that circ_0001367 was downregulated in glioma tissues and showed a close correlation with glioma patient survival. Functional assays demonstrated that upregulation of circ_0001367 could suppress the proliferation, migration and invasion of glioma cells in vitro and inhibit glioma growth in vivo. Furthermore, bioinformatics analysis, luciferase reporter assay and RNA immunoprecipitation assay indicated that circ_0001367 can serve as a sponge for miR-431 and that miR-431 acts as an oncogene by regulating neurexin 3 (NRXN3). In addition, rescue experiments verified that circ_0001367 could regulate both the expression and function of NRXN3 in a miR-431-dependent manner. In conclusion, circ_0001367 functions as an suppressor in glioma by targeting the miR-431/NRXN3 axis and may be a promising therapeutic target against gliomas.