Goniothalamin, a cytotoxic compound, isolated from Goniothalamus macrophyllus (Blume) Hook. f. & Thomson var. macrophyllus

Abstract

Bioassay guided fractionation by brine shrimp lethality test led to isolation of a cytotoxic compound, goniothalamin, from the root and stem of Goniothalamus macrophyllus (Blume) Hook. f. & Thomson var. macrophyllus. Goniothalamin showed a promising cytotoxicity (SRB assay) against colon cancer cell line (IC50 = 0.51±0.02 μg/ml), breast cancer cell lines (IC50= 0.95±0.02 μg/ml) and lung carcinoma (IC50 = 3.51± 0.03 μg/ml). LDH assay of goniothalamin suggested that it had no toxicity on cell membrane. Cytotoxic evaluation of goniothalamin to normal cell revealed moderate toxicity against skin fibroblast (IC50 = 26.73± 1.92 μg/ml) and human fibroblast (IC50 = 11.99±0.15 μg/ml).

Keywords

• Goniothalamus macrophyllus var. macrophyllus
• Goniothalamin
• Cytotoxic
• Brine shrimp lethality test
• SRB assay