Frontiers in Cellular Neuroscience (Feb 2015)
New Strategy in Spinal Cord Repair with Cryofrozen Primary Cell Cultures in Model In Vivo
Abstract
The approach to knowledge of the biological and functional characteristics of the OECs has prompted the development of studies where these cells have been implemented as a possible therapeutic strategy for injuries of the CNS. Furthermore, the production of different molecules favorable for axonal regeneration and the apparent ease of which the OECs penetrate the CNS, could be important characteristics in favor of the use of OECs. The purpose of the present study was to test the hypothesis that the transplant of cryopreserved OCEs plus aFGF + FG placed in the injured rat ́s, after a surgical trauma, could be capable to produce a regenerative response and to recuperate the motive function of the hind limbs. The investigation was approved by the Ethics Committee of the University of La Sabana. The OCEs cultures were obtained and purified from neonatal rats (10 days) and adult rats (5 months) from which allograft transplants (PNS) were extracted, nerve and external layer Viability  of olfactory bulb (CNS). The cultures were supplemented with DMEM and maintained at 37oC , 98% RH and 5% CO2. This process obtained an average of 8 x 105 cells and reaching a confluence of aproximately 80%. Subsequently, 2 x 105 cells were frozen at minus 192 oC (DMEM + FBS at 20% in DMSO at 10%). After approximately eight months we proceed to thaw cryogenic viales with the OECs 1.5 x 106 were used, injected in the center of the medullar cavity of the severe injury of the spinal cord of the experimental model. The rats that were subjected to surgery were maintained under observation for 42 days. The comparative study of treatments showed differences that are statistically significative with respect to the experimental group with the OECs cryopreserved + aFGF + FG reaching twelve points in the evaluation with the BBB scale. The results as show evidence that when evaluating the locomotor recuperation, after a medullar section in rats through the implantation of OECs of cryopreserved cells combined with aFGF + FG. In addition, the conclusion it is potencial use in reparative phenomena in the injured medullar tissue but making further studies necessary before considering their use.
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