Insights Into Walnut Lipid Metabolism From Metabolome and Transcriptome Analysis

Suxian Yan; Xingsu Wang; Chenkang Yang; Junyou Wang; Ying Wang; Bangbang Wu; Ling Qiao; Jiajia Zhao; Pourkheirandish Mohammad; Xingwei Zheng; Jianguo Xu; Huming Zhi; Jun Zheng

doi:10.3389/fgene.2021.715731

Frontiers in Genetics (Sep 2021)

Insights Into Walnut Lipid Metabolism From Metabolome and Transcriptome Analysis

Suxian Yan,
Xingsu Wang,
Chenkang Yang,
Junyou Wang,
Ying Wang,
Bangbang Wu,
Ling Qiao,
Jiajia Zhao,
Pourkheirandish Mohammad,
Xingwei Zheng,
Jianguo Xu,
Huming Zhi,
Jun Zheng

Affiliations

Suxian Yan: State Key Laboratory of Sustainable Dryland Agriculture, Institute of Wheat Research, Shanxi Agricultural University, Linfen, China
Xingsu Wang: College of Food Science, Shanxi Normal University, Linfen, China
Chenkang Yang: State Key Laboratory of Sustainable Dryland Agriculture, Institute of Wheat Research, Shanxi Agricultural University, Linfen, China
Junyou Wang: State Key Laboratory of Sustainable Dryland Agriculture, Institute of Wheat Research, Shanxi Agricultural University, Linfen, China
Ying Wang: State Key Laboratory of Sustainable Dryland Agriculture, Institute of Wheat Research, Shanxi Agricultural University, Linfen, China
Bangbang Wu: State Key Laboratory of Sustainable Dryland Agriculture, Institute of Wheat Research, Shanxi Agricultural University, Linfen, China
Ling Qiao: State Key Laboratory of Sustainable Dryland Agriculture, Institute of Wheat Research, Shanxi Agricultural University, Linfen, China
Jiajia Zhao: State Key Laboratory of Sustainable Dryland Agriculture, Institute of Wheat Research, Shanxi Agricultural University, Linfen, China
Pourkheirandish Mohammad: Plant Molecular Biology and Biotechnology Laboratory, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, VIC, Australia
Xingwei Zheng: State Key Laboratory of Sustainable Dryland Agriculture, Institute of Wheat Research, Shanxi Agricultural University, Linfen, China
Jianguo Xu: College of Food Science, Shanxi Normal University, Linfen, China
Huming Zhi: State Key Laboratory of Sustainable Dryland Agriculture, Institute of Wheat Research, Shanxi Agricultural University, Linfen, China
Jun Zheng: State Key Laboratory of Sustainable Dryland Agriculture, Institute of Wheat Research, Shanxi Agricultural University, Linfen, China

DOI: https://doi.org/10.3389/fgene.2021.715731
Journal volume & issue: Vol. 12

Abstract

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Walnut oil is an excellent source of essential fatty acids. Systematic evaluation of walnut lipids has significance for the development of the nutritional and functional value of walnut. Ultra-performance liquid chromatography/Orbitrap high-resolution mass spectrometry (UHPLC-Orbitrap HRMS) was used to characterize the lipids of walnut. A total of 525 lipids were detected and triacylglycerols (TG) (18:2/18:2/18:3) and diacylglycerols (DG) (18:2/18:2) were the main glycerolipids present. Essential fatty acids, such as linoleic acid and linolenic acid, were the main DG and TG fatty acid chains. Many types of phospholipids were observed with phosphatidic acid being present in the highest concentration (5.58%). Using a combination of metabolome and transcriptome analysis, the present study mapped the main lipid metabolism pathway in walnut. These results may provide a theoretical basis for further study and specific gene targets to enable the development of walnut with increased oil content and modified fatty acid composition.

Published in Frontiers in Genetics

ISSN: 1664-8021 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://journal.frontiersin.org/journal/genetics

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