Prevalence of Hepatopancreatic Parvovirus (HPV) in Penaeus monodon Postlarvae from Commercial Shrimp Hatcheries in Tamilnadu, Southeast Coast of India

Abstract

The prevalence of hepatopancreatic parvovirus (HPV) in Penaeus monodon postlarvae produced from commercial shrimp hatcheries in Tamilnadu, located along the southeast coast of India was studied during the period July 2002 to July 2003. A total of 1020 hatchery tanks rearing postlarvae were screened by microscopic method and polymerase chain reaction (PCR). Microscopic observation of Giemsa stained smear by rapid method or wet mount observation of squash preparation of hepatopancreas from postlarvae samples from the different tanks revealed a prevalence of 7.74% for HPV and 2.75% for dual infection with HPV and monodon baculovirus (MBV). PCR analysis of samples during the period of study showed a prevalence of 9.3% for HPV and 4.5% for dual infection with HPV and MBV.