Stem Cell Research (Mar 2016)

Generation of OCIAD1 inducible overexpression human embryonic stem cell line: BJNhem20-OCIAD1-Tet-On

  • Deeti K. Shetty,
  • Ronak Shetty,
  • Maneesha S. Inamdar

DOI
https://doi.org/10.1016/j.scr.2015.12.045
Journal volume & issue
Vol. 16, no. 2
pp. 246 – 248

Abstract

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Human embryonic stem cell line BJNhem20-OCIAD1-Tet-On was generated using non-viral method. The constructs pCAG-Tet-On and pTRE-Tight vector driving OCIAD1 expression were transfected using microporation procedure. pCAG-Tet-On cells can be used for inducible expression of any coding sequence cloned into pTRE-Tight vector. For example, in human embryonic stem cells, Tet-On system has been used to generate SOX2 overexpression cell line (Adachi et al., 2010).