Effect of Neem Leaf Extract (Azadirachta indica) on c-MycOncogene Expression in 4T1 Breast Cancer Cells of BALB/c Mice

Abstract

Objective: Breast cancer is the most common cause of cancer-related deaths in women both worldwide and in Malaysia. Azadirachta indica (A. Juss), commonly known as neem, is one of the most versatile medicinal plants that has gained worldwide prominence due to its medicinal properties. However, the anticancer effect of ethanolic neem leaf extract against breast cancer has not been documented. The purpose of the present study is to investigate the effect of neem leaf extract on c-Myc oncogene expression in 4T1 breast cancer BALB/c mice.Materials and Methods: In this experimental study, A total of 48 female BALB/c mice were divided randomly into four groups of 12 mice per group: i.cancer control (CC) treated with 0.5% Tween 20 in PBS, ii. 0.5 μg/mL tamoxifen citrate (CT), iii. 250 mg/kg neem leaf extract (C250), and iv. 500 mg/kg neem leaf extract (C500). In situ reverse transcription polymerase chain reaction (in situ RT-PCR) was applied to evaluate suppression of c-Myc oncogene expression in breast cancer tissue.Results: The C500 group showed significant (p<0.05) suppression of c-Myc oncogene expression compared to the CC group.Conclusion: c-Myc was found to be down regulated under the effect of 500 mg/kg ethanolicneem leaf extract.

Keywords

• Breast Cancer
• c-Myc Gene
• Primed In situ Labelling