Newly synthesized carbocyanine fluorescent probes, their characteristics and behavior in proliferating cultures

Biopolymers and Cell. 2009;25(6):484-490 DOI 10.7124/bc.0007FB


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Journal Title: Biopolymers and Cell

ISSN: 0233-7657 (Print); 1993-6842 (Online)

Publisher: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine

LCC Subject Category: Science: Biology (General): Genetics

Country of publisher: Ukraine

Language of fulltext: English

Full-text formats available: PDF



Grischenko V. I.

Malyukin Yu. V.

Pavlovich E. V.

Borovoy I. A.

Goncharuk E. I.


Double blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 8 weeks


Abstract | Full Text

Aim. To study possibile application of C2, C9, C18 and JC-1 carbocyanine fluorescent dyes for cell culture characterization. Methods. Morphological methods, fluorescence-activated cell sorting (FACS) analysis, luminescent microscopy were used. Results. The studied carbocyanine probes were shown to be preserved in dividing cells for at least 4 duplications. It was found that carbocyanine probe JC-1 did not transit from cell to cell under combined culturing of labeled and non-labeled cells. Conclusions. The paper covers the use of carbocyanine fluorescent probes for long-term culturing of cell lines. Probes C9 and JC-1 were optimal for the proliferative culture observation, allowing to trace mitochondrial functional state