Journal Title: Biopolymers and Cell
ISSN: 0233-7657 (Print); 1993-6842 (Online)
Publisher: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine
LCC Subject Category: Science: Biology (General): Genetics
Country of publisher: Ukraine
Language of fulltext: English
Full-text formats available: PDF
Grischenko V. I.
Malyukin Yu. V.
Pavlovich E. V.
Borovoy I. A.
Goncharuk E. I.
Abstract | Full Text
Aim. To study possibile application of C2, C9, C18 and JC-1 carbocyanine fluorescent dyes for cell culture characterization. Methods. Morphological methods, fluorescence-activated cell sorting (FACS) analysis, luminescent microscopy were used. Results. The studied carbocyanine probes were shown to be preserved in dividing cells for at least 4 duplications. It was found that carbocyanine probe JC-1 did not transit from cell to cell under combined culturing of labeled and non-labeled cells. Conclusions. The paper covers the use of carbocyanine fluorescent probes for long-term culturing of cell lines. Probes C9 and JC-1 were optimal for the proliferative culture observation, allowing to trace mitochondrial functional state