Newly synthesized carbocyanine fluorescent probes, their characteristics and behavior in proliferating cultures

Biopolymers and Cell. 2009;25(6):484-490 DOI 10.7124/bc.0007FB

 

Journal Homepage

Journal Title: Biopolymers and Cell

ISSN: 0233-7657 (Print); 1993-6842 (Online)

Publisher: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine

LCC Subject Category: Science: Biology (General): Genetics

Country of publisher: Ukraine

Language of fulltext: English

Full-text formats available: PDF

 

AUTHORS

Grischenko V. I.
Malyukin Yu. V.
Pavlovich E. V.
Borovoy I. A.
Goncharuk E. I.

EDITORIAL INFORMATION

Double blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 8 weeks

 

Abstract | Full Text

Aim. To study possibile application of C2, C9, C18 and JC-1 carbocyanine fluorescent dyes for cell culture characterization. Methods. Morphological methods, fluorescence-activated cell sorting (FACS) analysis, luminescent microscopy were used. Results. The studied carbocyanine probes were shown to be preserved in dividing cells for at least 4 duplications. It was found that carbocyanine probe JC-1 did not transit from cell to cell under combined culturing of labeled and non-labeled cells. Conclusions. The paper covers the use of carbocyanine fluorescent probes for long-term culturing of cell lines. Probes C9 and JC-1 were optimal for the proliferative culture observation, allowing to trace mitochondrial functional state