The study of the therapeutic effectiveness of the associated inactivated and subunit vaccines based on Candida albicans and Candida tropicalis fungi

Abstract

Candidiasis can be in various forms, the most dangerous of them are systemic and visceral candidiases. Many researchers believe that using drugs that are able to stimulate a protective immune response against candidal infections, i.e. immunobiological drugs, is a promising direction in the fight against candidiasis, and that these drugs are an alternative to antifungal agents. The aim of the work was to compare the therapeutic properties of the inactivated and subunit vaccines of cells of C. albicans and C. tropicalis fungi. Previously, animals were infected with the candidal infection. In 5 days, after infection the vaccines studied were injected to mice intramuscularly in the volume of 0.2 ml in the upper part of the rear right paw. In 14 days, these vaccines were injected to mice in the volume of 0.2 ml in the upper part of the rear left paw. In 14 days, the body protection functions were determined by the titer of C. albicans specific antibodies when performing the enzyme-linked immunosorbent assay (ELISA). According to the results of the research conducted it has been found that the use of the inactivated and subunit vaccines stimulates the eight times growth of the titers. Therefore, both vaccines are effective. However, the subunit vaccine has the maximum purification from ballast substances; therefore, it is promising to use exactly this vaccine for further studies.