Frontiers in Immunology (Sep 2022)
Clec7a expression in inflammatory macrophages orchestrates progression of acute kidney injury
- Yaqiong Wang,
- Yaqiong Wang,
- Yaqiong Wang,
- Yaqiong Wang,
- Yaqiong Wang,
- Xianzhe Li,
- Xianzhe Li,
- Xianzhe Li,
- Xianzhe Li,
- Xianzhe Li,
- Xialian Xu,
- Xialian Xu,
- Xialian Xu,
- Xialian Xu,
- Xialian Xu,
- Jinbo Yu,
- Jinbo Yu,
- Jinbo Yu,
- Jinbo Yu,
- Jinbo Yu,
- Xiaohong Chen,
- Xiaohong Chen,
- Xiaohong Chen,
- Xiaohong Chen,
- Xiaohong Chen,
- Xuesen Cao,
- Xuesen Cao,
- Xuesen Cao,
- Xuesen Cao,
- Xuesen Cao,
- Jianzhou Zou,
- Jianzhou Zou,
- Jianzhou Zou,
- Jianzhou Zou,
- Jianzhou Zou,
- Bo Shen,
- Bo Shen,
- Bo Shen,
- Bo Shen,
- Bo Shen,
- Xiaoqiang Ding,
- Xiaoqiang Ding,
- Xiaoqiang Ding,
- Xiaoqiang Ding,
- Xiaoqiang Ding
Affiliations
- Yaqiong Wang
- Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China
- Yaqiong Wang
- Shanghai Medical Center of Kidney, Shanghai, China
- Yaqiong Wang
- Department of Nephrology, Shanghai Institute of Kidney and Dialysis, Shanghai, China
- Yaqiong Wang
- Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China
- Yaqiong Wang
- Hemodialysis Quality Control Center of Shanghai, Shanghai, China
- Xianzhe Li
- Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China
- Xianzhe Li
- Shanghai Medical Center of Kidney, Shanghai, China
- Xianzhe Li
- Department of Nephrology, Shanghai Institute of Kidney and Dialysis, Shanghai, China
- Xianzhe Li
- Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China
- Xianzhe Li
- Hemodialysis Quality Control Center of Shanghai, Shanghai, China
- Xialian Xu
- Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China
- Xialian Xu
- Shanghai Medical Center of Kidney, Shanghai, China
- Xialian Xu
- Department of Nephrology, Shanghai Institute of Kidney and Dialysis, Shanghai, China
- Xialian Xu
- Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China
- Xialian Xu
- Hemodialysis Quality Control Center of Shanghai, Shanghai, China
- Jinbo Yu
- Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China
- Jinbo Yu
- Shanghai Medical Center of Kidney, Shanghai, China
- Jinbo Yu
- Department of Nephrology, Shanghai Institute of Kidney and Dialysis, Shanghai, China
- Jinbo Yu
- Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China
- Jinbo Yu
- Hemodialysis Quality Control Center of Shanghai, Shanghai, China
- Xiaohong Chen
- Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China
- Xiaohong Chen
- Shanghai Medical Center of Kidney, Shanghai, China
- Xiaohong Chen
- Department of Nephrology, Shanghai Institute of Kidney and Dialysis, Shanghai, China
- Xiaohong Chen
- Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China
- Xiaohong Chen
- Hemodialysis Quality Control Center of Shanghai, Shanghai, China
- Xuesen Cao
- Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China
- Xuesen Cao
- Shanghai Medical Center of Kidney, Shanghai, China
- Xuesen Cao
- Department of Nephrology, Shanghai Institute of Kidney and Dialysis, Shanghai, China
- Xuesen Cao
- Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China
- Xuesen Cao
- Hemodialysis Quality Control Center of Shanghai, Shanghai, China
- Jianzhou Zou
- Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China
- Jianzhou Zou
- Shanghai Medical Center of Kidney, Shanghai, China
- Jianzhou Zou
- Department of Nephrology, Shanghai Institute of Kidney and Dialysis, Shanghai, China
- Jianzhou Zou
- Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China
- Jianzhou Zou
- Hemodialysis Quality Control Center of Shanghai, Shanghai, China
- Bo Shen
- Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China
- Bo Shen
- Shanghai Medical Center of Kidney, Shanghai, China
- Bo Shen
- Department of Nephrology, Shanghai Institute of Kidney and Dialysis, Shanghai, China
- Bo Shen
- Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China
- Bo Shen
- Hemodialysis Quality Control Center of Shanghai, Shanghai, China
- Xiaoqiang Ding
- Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China
- Xiaoqiang Ding
- Shanghai Medical Center of Kidney, Shanghai, China
- Xiaoqiang Ding
- Department of Nephrology, Shanghai Institute of Kidney and Dialysis, Shanghai, China
- Xiaoqiang Ding
- Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China
- Xiaoqiang Ding
- Hemodialysis Quality Control Center of Shanghai, Shanghai, China
- DOI
- https://doi.org/10.3389/fimmu.2022.1008727
- Journal volume & issue
-
Vol. 13
Abstract
Acute kidney injury (AKI) is associated with high risk of mortality, post-disease renal fibrosis, kidney dysfunction and renal failure. Renal macrophages play a key role in the pathogenesis (M1 subpopulation), healing and remodeling (M2 subpopulation) in AKI and, thus, have been a promising target for clinical treatment of AKI. Here, in a mouse renal ischemia/reperfusion injury (IRI) model for AKI, we showed that renal macrophages could be further classified into Clec7a+ M1 macrophages, Clec7a- M1 macrophages, Clec7a+ M2 macrophages and Clec7a- M2 macrophages, representing distinct macrophage populations with different functionality. Interestingly, Clec7a+ M1 macrophages exhibited potent pro-inflammatory and phagocytic effects compared to Clec7a- M1 macrophages, while Clec7a- M2 macrophages exhibited better proliferating and migrating potential, which is critical for their role in tissue repairing after injury. These data from mice were further strengthened by bioinformatics analyses using published database. In vivo, combined expression of Clec7a in M1 macrophages and depletion of Clec7a in M2 macrophages significantly improved the renal function after IRI-AKI. Together, our data suggest that Clec7a is crucial for the fine regulation of macrophage phenotype during AKI and could be a novel target for boosting clinical therapy.
Keywords
- acute kidney injury (AKI)
- ischemia/reperfusion injury (IRI)
- macrophages
- Clec7a
- M1 macrophages
- M2 macrophages
