Improving Tree Seedling Quality Using Humates Combined with Bacteria to Address Decarbonization Challenges through Forest Restoration
Aleksey Nazarov,
Sergey Chetverikov,
Maxim Timergalin,
Ruslan Ivanov,
Nadezhda Ryazanova,
Zinnur Shigapov,
Iren Tuktarova,
Ruslan Urazgildin,
Guzel Kudoyarova
Affiliations
Aleksey Nazarov
Department of Environment and Rational Use of Natural Resources, Faculty of Business Ecosystem and Creative Technologies, Ufa State Petroleum Technological University, ul. Kosmonavtov 1, 450064 Ufa, Russia
Sergey Chetverikov
Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, Prospekt Oktyabrya 69, 450054 Ufa, Russia
Maxim Timergalin
Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, Prospekt Oktyabrya 69, 450054 Ufa, Russia
Ruslan Ivanov
Department of Environment and Rational Use of Natural Resources, Faculty of Business Ecosystem and Creative Technologies, Ufa State Petroleum Technological University, ul. Kosmonavtov 1, 450064 Ufa, Russia
Nadezhda Ryazanova
Department of Environment and Rational Use of Natural Resources, Faculty of Business Ecosystem and Creative Technologies, Ufa State Petroleum Technological University, ul. Kosmonavtov 1, 450064 Ufa, Russia
Zinnur Shigapov
South Ural Botanical Garden-Institute, Ufa Federal Research Center, Russian Academy of Sciences, 450080 Ufa, Russia
Iren Tuktarova
Department of Environment and Rational Use of Natural Resources, Faculty of Business Ecosystem and Creative Technologies, Ufa State Petroleum Technological University, ul. Kosmonavtov 1, 450064 Ufa, Russia
Ruslan Urazgildin
Department of Environment and Rational Use of Natural Resources, Faculty of Business Ecosystem and Creative Technologies, Ufa State Petroleum Technological University, ul. Kosmonavtov 1, 450064 Ufa, Russia
Guzel Kudoyarova
Department of Environment and Rational Use of Natural Resources, Faculty of Business Ecosystem and Creative Technologies, Ufa State Petroleum Technological University, ul. Kosmonavtov 1, 450064 Ufa, Russia
Improving the quality of tree planting material for carbon sequestration through reforestation can help solve environmental problems, including the need to reduce the concentration of carbon dioxide in the atmosphere. The purpose of this study was to investigate the possibility of using humic substances in combination with rhizosphere microorganisms Pseudomonas protegens DA1.2 and Pseudomonas sp. 4CH as a means to stimulate the growth of seedlings of pine, poplar, large-leaved linden, red oak, horse chestnut, and rowan. Humic substances stimulated the growth of shoots and roots of pine, large-leaved linden, and horse chestnut seedlings. The effects of bacteria depended on both plant and bacteria species: Pseudomonas protegens DA1.2 showed a higher stimulatory effect than Pseudomonas sp. 4CH on pine and linden, and Pseudomonas sp. 4CH was more effective in the case of chestnut. An additive effect of humates and Pseudomonas protegens DA1.2 on the growth rate of pine and linden saplings was discovered. Poplar, red oak, and rowan seedlings were unresponsive to the treatments. The growth-stimulating effects of the treatments are discussed in connection with the changes in carbon, chlorophyll, and nitrogen contents in plants. The results show the need for further research in bacterial species capable of stimulating the growth of plant species that were unresponsive in the present experiments.