Reversible induction of mitophagy by an optogenetic bimodular system

Pasquale D’Acunzo; Flavie Strappazzon; Ignazio Caruana; Giacomo Meneghetti; Anthea Di Rita; Luca Simula; Gerrit Weber; Francesca Del Bufalo; Luisa Dalla Valle; Silvia Campello; Franco Locatelli; Francesco Cecconi

doi:10.1038/s41467-019-09487-1

Nature Communications (Apr 2019)

Reversible induction of mitophagy by an optogenetic bimodular system

Pasquale D’Acunzo,
Flavie Strappazzon,
Ignazio Caruana,
Giacomo Meneghetti,
Anthea Di Rita,
Luca Simula,
Gerrit Weber,
Francesca Del Bufalo,
Luisa Dalla Valle,
Silvia Campello,
Franco Locatelli,
Francesco Cecconi

Affiliations

Pasquale D’Acunzo: Department of Paediatric Haematology, Oncology and Cell and Gene Therapy, IRCCS Bambino Gesù Children’s Hospital
Flavie Strappazzon: IRCCS Fondazione Santa Lucia
Ignazio Caruana: Department of Paediatric Haematology, Oncology and Cell and Gene Therapy, IRCCS Bambino Gesù Children’s Hospital
Giacomo Meneghetti: Department of Biology, University of Padova
Anthea Di Rita: IRCCS Fondazione Santa Lucia
Luca Simula: Department of Paediatric Haematology, Oncology and Cell and Gene Therapy, IRCCS Bambino Gesù Children’s Hospital
Gerrit Weber: Department of Paediatric Haematology, Oncology and Cell and Gene Therapy, IRCCS Bambino Gesù Children’s Hospital
Francesca Del Bufalo: Department of Paediatric Haematology, Oncology and Cell and Gene Therapy, IRCCS Bambino Gesù Children’s Hospital
Luisa Dalla Valle: Department of Biology, University of Padova
Silvia Campello: IRCCS Fondazione Santa Lucia
Franco Locatelli: Department of Paediatric Haematology, Oncology and Cell and Gene Therapy, IRCCS Bambino Gesù Children’s Hospital
Francesco Cecconi: Department of Paediatric Haematology, Oncology and Cell and Gene Therapy, IRCCS Bambino Gesù Children’s Hospital

DOI: https://doi.org/10.1038/s41467-019-09487-1
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 13

Abstract

Read online

Autophagic degradation of mitochondria (mitophagy) is a key quality control mechanism in cellular homeostasis, and its misregulation is involved in neurodegenerative diseases. Here the authors develop an optogenetic system for reversible induction of mitophagy and validate its use in cell culture and zebrafish embryos.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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