Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triple-negative Breast Cancer Cells

Abstract

Objective To investigate the expression of circular RNA ciRS-7 in triple-negative breast cancer (TNBC) and its effect on invasion and migration. Methods The expression levels of ciRS-7 in TNBC tissues and cells were detected by qRT-PCR, and its relationship with clinicopathological parameters of breast cancer patients was further analyzed. Scratch and Transwell assays were used to detect the migration and invasion of MDA-MB-231 and BT-549 cells after silencing ciRS-7. Animal experiment was performed to verify the above findings in vivo. Results The relative expression of ciRS-7 in TNBC tissues was (6.52±0.38), significantly higher than Luminal type (1.56±0.17) (P 0.05). After silencing ciRS-7, the migration and invasion of MDA-MB-231 and BT-549 cells were significantly reduced (all P < 0.05). The number of lung metastasis clones was significantly reduced in ciRS-7 knockdown group (P < 0.05). Conclusion ciRS-7 is highly expressed in TNBC and may enhance the invasion and migration of TNBC cells.

Keywords

• cirs-7
• triple negative breast cancer (tnbc)
• migration
• invasion