Antioxidant property of aminophylline in rat brain

Abstract

Background: Free radicals being considered an important component of secondary damage during ischemia, trauma, and neurodegenerative diseases in the CNS. Aminophylline has been reported to possess free radical scavenging effect in lung tissue. But there is no information regarding aminophylline's neuroprotective effect against lipid peroxidation in the brain. Aim: The present study was to locate the region of brain that is particularly susceptible to oxidative damage and also aimed at investigating the antioxidant action of aminophylline in rat brain homogenates against in vitro induced lipid peroxidation. Method: Male Wister rats (n = 34) were randomly selected and divided into three groups. The whole rat brains all rats were homogenized in 1:10 cold Tris–HCl buffer by using homogenizer. Peroxidation was induced with ferrous iron (Fe2+), ascorbate and hydrogen peroxide (H2O2) in Group I (n = 12). Aminophylline was added into the reaction mixtures just before the induction of peroxidation in Group III (n = 12). Result: The highest lipid peroxidation was obtained with Fe2+, Ascorbate and H202-induced group and aminophylline showed definite free radical scavenging effect on rat brain. Conclusion: Analysis of the results demonstrated that aminophylline has no antioxidant effect on rat brain and spinal cord homogenates in vitro. Further in vitro and in vivo studies are needed to evaluate the free radical scavenging effect of aminophylline.

Keywords

• Aminophylline
• Antioxidant property
• Oxidative stress
• lipid peroxidation