Журнал микробиологии, эпидемиологии и иммунобиологии (Nov 2019)

Development of the scheme of obtaining antibodies to the ribonucleoprotein of attenuated rabies virus

  • Yu. K. Gavrilova,
  • S. V. Generalov,
  • M. N. Kireev,
  • N. A. Sharapova,
  • E. G. Abramova,
  • L. V. Savitskaya,
  • M. V. Ovchinnikova,
  • T. Yu. Kirillova,
  • A. P. Semakova

DOI
https://doi.org/10.36233/0372-9311-2019-5-3-8
Journal volume & issue
Vol. 0, no. 5
pp. 3 – 8

Abstract

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Aim. Isolation of ribonucleoprotein (RNP) of an attenuated rabies virus, develop schemes for immunizing animals with RNP-based preparations and determine the most effective scheme that allows obtaining serum with a high antibody titer to the RNP.Materials and methods. We used the transplantable cell line Vero, the strain rabies virus «Moscow 3253 Vero», adapted for reproduction on Vero, rabbits of the chinchilla breed. In order to obtain serums containing antibodies to the RNP of the rabies virus, experimental schemes have been proposed for immunizing animals with RNP, including with adjuvants: polyoxidonium and colloidal gold. The dynamics of the accumulation of antibodies to the RNP of the rabies virus in the blood serum of experimental animals was studied by dot-immunoassay.Results. The target component (RNP of the rabies virus) was isolated directly from the cytoplasm of the Vero cell culture infected with the rabies virus according to the modified M. Dastkhosh (2014) method, lyophilized and used in the development of preparations for immunizing experimental animals. In the study of the dynamics of the formation of antibodies to RNP of the rabies virus by the method of dot-immunoassay, the effectiveness of an adjuvant is established — colloidal gold nanoparticles ranging in size from 15 to 17 nm, the use of which makes it possible to increase the antibody titer by 2 times.Conclusion. The results obtained are of interest for further research related to the design of diagnostic products and the development of methodological techniques using such preparations.

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