Nature Communications (Sep 2022)
Cost-effective methylome sequencing of cell-free DNA for accurately detecting and locating cancer
- Mary L. Stackpole,
- Weihua Zeng,
- Shuo Li,
- Chun-Chi Liu,
- Yonggang Zhou,
- Shanshan He,
- Angela Yeh,
- Ziye Wang,
- Fengzhu Sun,
- Qingjiao Li,
- Zuyang Yuan,
- Asli Yildirim,
- Pin-Jung Chen,
- Paul Winograd,
- Benjamin Tran,
- Yi-Te Lee,
- Paul Shize Li,
- Zorawar Noor,
- Megumi Yokomizo,
- Preeti Ahuja,
- Yazhen Zhu,
- Hsian-Rong Tseng,
- James S. Tomlinson,
- Edward Garon,
- Samuel French,
- Clara E. Magyar,
- Sarah Dry,
- Clara Lajonchere,
- Daniel Geschwind,
- Gina Choi,
- Sammy Saab,
- Frank Alber,
- Wing Hung Wong,
- Steven M. Dubinett,
- Denise R. Aberle,
- Vatche Agopian,
- Steven-Huy B. Han,
- Xiaohui Ni,
- Wenyuan Li,
- Xianghong Jasmine Zhou
Affiliations
- Mary L. Stackpole
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Weihua Zeng
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Shuo Li
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Chun-Chi Liu
- EarlyDiagnostics, Inc.
- Yonggang Zhou
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Shanshan He
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Angela Yeh
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Ziye Wang
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Fengzhu Sun
- Department of Quantitative and Computational Biology, University of Southern California
- Qingjiao Li
- The Eighth Affiliated Hospital, Sun Yat-Sen University
- Zuyang Yuan
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Asli Yildirim
- Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles
- Pin-Jung Chen
- Department of Surgery, University of California at Los Angeles
- Paul Winograd
- Department of Surgery, University of California at Los Angeles
- Benjamin Tran
- Department of Surgery, University of California at Los Angeles
- Yi-Te Lee
- Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California at Los Angeles
- Paul Shize Li
- Westlake High School
- Zorawar Noor
- Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Megumi Yokomizo
- Department of Radiological Sciences, David Geffen School of Medicine, University of California at Los Angeles
- Preeti Ahuja
- Department of Radiological Sciences, David Geffen School of Medicine, University of California at Los Angeles
- Yazhen Zhu
- Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California at Los Angeles
- Hsian-Rong Tseng
- Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California at Los Angeles
- James S. Tomlinson
- Department of Surgery, University of California at Los Angeles
- Edward Garon
- Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Samuel French
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Clara E. Magyar
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Sarah Dry
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Clara Lajonchere
- Jonsson Comprehensive Cancer Center, University of California at Los Angeles
- Daniel Geschwind
- Institute for Precision Health, University of California at Los Angeles
- Gina Choi
- Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Sammy Saab
- Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Frank Alber
- Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles
- Wing Hung Wong
- Department of Statistics, Stanford University
- Steven M. Dubinett
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Denise R. Aberle
- Department of Radiological Sciences, David Geffen School of Medicine, University of California at Los Angeles
- Vatche Agopian
- Department of Surgery, University of California at Los Angeles
- Steven-Huy B. Han
- Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Xiaohui Ni
- EarlyDiagnostics, Inc.
- Wenyuan Li
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- Xianghong Jasmine Zhou
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
- DOI
- https://doi.org/10.1038/s41467-022-32995-6
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 12
Abstract
Early cancer detection by cell-free DNA (cfDNA) is challenged by the low amount of tumour DNA in cfDNA, tumour heterogeneity and the small patient cohorts. Here, the authors develop a method, cfMethyl-Seq, for cost-effective methylome profiling of cfDNA and for detecting and locating cancer.
