In Vitro Activity of Essential Oils from Piper Species (Piperaceae) against Tachyzoites of Toxoplasma gondii

Adalberto Alves Pereira Filho; Mariana Maciel Cunha; Mariana Alves Stanton; Lydia Fumiko Yamaguchi; Massuo Jorge Kato; Érica S. Martins-Duarte

doi:10.3390/metabo13010095

Metabolites (Jan 2023)

In Vitro Activity of Essential Oils from Piper Species (Piperaceae) against Tachyzoites of Toxoplasma gondii

Adalberto Alves Pereira Filho,
Mariana Maciel Cunha,
Mariana Alves Stanton,
Lydia Fumiko Yamaguchi,
Massuo Jorge Kato,
Érica S. Martins-Duarte

Affiliations

Adalberto Alves Pereira Filho: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil
Mariana Maciel Cunha: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil
Mariana Alves Stanton: Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508-000, São Paulo, Brazil
Lydia Fumiko Yamaguchi: Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508-000, São Paulo, Brazil
Massuo Jorge Kato: Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508-000, São Paulo, Brazil
Érica S. Martins-Duarte: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil

DOI: https://doi.org/10.3390/metabo13010095
Journal volume & issue: Vol. 13, no. 1
p. 95

Abstract

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Toxoplasmosis is a tropical and neglected disease caused by the parasitic protozoa Toxplasma gondii. Conventional treatment with sulfadiazine and pyrimethamine plus folinic acid, has some drawbacks, such as inefficacy in the chronic phase, toxic side effects, and potential cases of resistance have been observed. In this study, the activity of essential oils (EOs) from three Piper species and their main constituents, including α-Pinene (Piper lindbergii and P. cernuum), β-Pinene (P. cernuum), and dillapiole (P. aduncum), were evaluated against tachyzoites of T. gondii. α-Pinene was more active [(IC50 0.3265 (0.2958 to 0.3604) μg/mL)] against tachyzoites than P. lindbergii EO [0.8387 (0.6492 to 1.084) μg/mL]. Both α-Pinene and P. lindbergii EO exhibited low cytotoxicity against NHDF cells, with CC50 41.37 (37.64 to 45.09) µg/mL and 83.80 (75.42 to 91.34) µg/mL, respectively, suggesting they could be of potential use against toxoplasmosis.

Published in Metabolites

ISSN: 2218-1989 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.mdpi.com/journal/metabolites

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