Induction of Inflammatory Macrophages in the Gut and Extra-Gut Tissues by Colitis-Mediated Escherichia coli
Houbao Qi,
Yunhuan Gao,
Yuanyuan Li,
Jianmei Wei,
Xiaomin Su,
Chunze Zhang,
Yingquan Liu,
Hua Zhu,
Lei Sui,
Yanwen Xiong,
Xi Yang,
Yanmei Xu,
Yuan Zhang,
Rongcun Yang
Affiliations
Houbao Qi
State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China; Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin 300071, China; Department of Immunology, Nankai University School of Medicine, Nankai University, Tianjin 300071, China
Yunhuan Gao
State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China; Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin 300071, China; Department of Immunology, Nankai University School of Medicine, Nankai University, Tianjin 300071, China
Yuanyuan Li
State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China; Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin 300071, China; Department of Immunology, Nankai University School of Medicine, Nankai University, Tianjin 300071, China
Jianmei Wei
State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China; Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin 300071, China; Department of Immunology, Nankai University School of Medicine, Nankai University, Tianjin 300071, China
Xiaomin Su
State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China; Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin 300071, China; Department of Immunology, Nankai University School of Medicine, Nankai University, Tianjin 300071, China
Chunze Zhang
Department of Colorectal Surgery, Tianjin Union Medical Center, Tianjin 300121, China
Yingquan Liu
State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China; Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin 300071, China; Department of Immunology, Nankai University School of Medicine, Nankai University, Tianjin 300071, China
Hua Zhu
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) Comparative Medical Center, Peking Union Medical College (PUMC), Beijing 100021, China
Lei Sui
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) Comparative Medical Center, Peking Union Medical College (PUMC), Beijing 100021, China
Yanwen Xiong
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease, Control and Prevention, 155 Changbai Road, Changping District, Beijing 102206, China
Xi Yang
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease, Control and Prevention, 155 Changbai Road, Changping District, Beijing 102206, China
Yanmei Xu
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease, Control and Prevention, 155 Changbai Road, Changping District, Beijing 102206, China
Yuan Zhang
State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China; Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin 300071, China; Department of Immunology, Nankai University School of Medicine, Nankai University, Tianjin 300071, China
Rongcun Yang
State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China; Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin 300071, China; Department of Immunology, Nankai University School of Medicine, Nankai University, Tianjin 300071, China; Corresponding author
Summary: Inflammatory macrophages play a critical role in gut and extra-gut inflammatory disorders, which may be promoted through the dysbiosis of gut microbiota. However, it is poorly understood how gut microbiota affect inflammatory macrophages. Here, we found that increased Escherichia coli (E. coli) in inflamed colon may induce inflammatory macrophages in gut and extra-gut tissues. These E. coli are different from other commensal and pathogenic E. coli in genomic components and also in ability to induce inflammatory responses. Dominant E. coli from colitic tissues induce gut inflammatory macrophages through a regulating network consisted of IL-18, IFN-γ, IL-12, and IL-22 in gut tissues. These E. coli also directly activate macrophages. Cytosolic inflammasome components PCKδ, NLRC4, caspase8, and caspase1/11 are involved in E. coli-mediated activation in both gut epithelial cells and macrophages. These disclose a novel mechanism for how dysbiosis of gut microbiota in colitis cause inflammatory macrophages related to multiple diseases. : Biological Sciences; Pathophysiology; Immunology; Microbiology Subject Areas: Biological Sciences, Pathophysiology, Immunology, Microbiology
