Nature Communications (Aug 2022)
Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers
- Susannah Holmes,
- Henry J. Kirkwood,
- Richard Bean,
- Klaus Giewekemeyer,
- Andrew V. Martin,
- Marjan Hadian-Jazi,
- Max O. Wiedorn,
- Dominik Oberthür,
- Hugh Marman,
- Luigi Adriano,
- Nasser Al-Qudami,
- Saša Bajt,
- Imrich Barák,
- Sadia Bari,
- Johan Bielecki,
- Sandor Brockhauser,
- Mathew A. Coleman,
- Francisco Cruz-Mazo,
- Cyril Danilevski,
- Katerina Dörner,
- Alfonso M. Gañán-Calvo,
- Rita Graceffa,
- Hans Fanghor,
- Michael Heymann,
- Matthias Frank,
- Alexander Kaukher,
- Yoonhee Kim,
- Bostjan Kobe,
- Juraj Knoška,
- Torsten Laurus,
- Romain Letrun,
- Luis Maia,
- Marc Messerschmidt,
- Markus Metz,
- Thomas Michelat,
- Grant Mills,
- Serguei Molodtsov,
- Diana C. F. Monteiro,
- Andrew J. Morgan,
- Astrid Münnich,
- Gisel E. Peña Murillo,
- Gianpietro Previtali,
- Adam Round,
- Tokushi Sato,
- Robin Schubert,
- Joachim Schulz,
- Megan Shelby,
- Carolin Seuring,
- Jonas A. Sellberg,
- Marcin Sikorski,
- Alessandro Silenzi,
- Stephan Stern,
- Jola Sztuk-Dambietz,
- Janusz Szuba,
- Martin Trebbin,
- Patrick Vagovic,
- Thomas Ve,
- Britta Weinhausen,
- Krzysztof Wrona,
- Paul Lourdu Xavier,
- Chen Xu,
- Oleksandr Yefanov,
- Keith A. Nugent,
- Henry N. Chapman,
- Adrian P. Mancuso,
- Anton Barty,
- Brian Abbey,
- Connie Darmanin
Affiliations
- Susannah Holmes
- Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University
- Henry J. Kirkwood
- European XFEL
- Richard Bean
- European XFEL
- Klaus Giewekemeyer
- European XFEL
- Andrew V. Martin
- School of Science, RMIT University
- Marjan Hadian-Jazi
- Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University
- Max O. Wiedorn
- Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY
- Dominik Oberthür
- Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY
- Hugh Marman
- Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University
- Luigi Adriano
- Deutsches Elektronen-Synchrotron DESY
- Nasser Al-Qudami
- European XFEL
- Saša Bajt
- Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY
- Imrich Barák
- Institute of Molecular Biology, SAS
- Sadia Bari
- Deutsches Elektronen-Synchrotron DESY
- Johan Bielecki
- European XFEL
- Sandor Brockhauser
- European XFEL
- Mathew A. Coleman
- Lawrence Livermore National Laboratory
- Francisco Cruz-Mazo
- Dept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla
- Cyril Danilevski
- European XFEL
- Katerina Dörner
- European XFEL
- Alfonso M. Gañán-Calvo
- Dept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla
- Rita Graceffa
- European XFEL
- Hans Fanghor
- European XFEL
- Michael Heymann
- Institute of Biomaterials and Biomolecular Systems, University of Stuttgart
- Matthias Frank
- Lawrence Livermore National Laboratory
- Alexander Kaukher
- European XFEL
- Yoonhee Kim
- European XFEL
- Bostjan Kobe
- School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience and Australian Infectious Diseases Research Centre, University of Queensland
- Juraj Knoška
- Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY
- Torsten Laurus
- Deutsches Elektronen-Synchrotron DESY
- Romain Letrun
- European XFEL
- Luis Maia
- European XFEL
- Marc Messerschmidt
- School of Molecular Science, Arizona State University
- Markus Metz
- Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY
- Thomas Michelat
- European XFEL
- Grant Mills
- European XFEL
- Serguei Molodtsov
- European XFEL
- Diana C. F. Monteiro
- The Hamburg Centre for Ultrafast Imaging
- Andrew J. Morgan
- Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY
- Astrid Münnich
- European XFEL
- Gisel E. Peña Murillo
- Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY
- Gianpietro Previtali
- European XFEL
- Adam Round
- European XFEL
- Tokushi Sato
- European XFEL
- Robin Schubert
- European XFEL
- Joachim Schulz
- European XFEL
- Megan Shelby
- Lawrence Livermore National Laboratory
- Carolin Seuring
- Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY
- Jonas A. Sellberg
- Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology
- Marcin Sikorski
- European XFEL
- Alessandro Silenzi
- European XFEL
- Stephan Stern
- European XFEL
- Jola Sztuk-Dambietz
- European XFEL
- Janusz Szuba
- European XFEL
- Martin Trebbin
- Institute of Experimental Physics, TU Bergakademie Freiberg
- Patrick Vagovic
- European XFEL
- Thomas Ve
- Institute for Glycomics, Griffith University
- Britta Weinhausen
- European XFEL
- Krzysztof Wrona
- European XFEL
- Paul Lourdu Xavier
- European XFEL
- Chen Xu
- European XFEL
- Oleksandr Yefanov
- Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY
- Keith A. Nugent
- Department of Quantum Science and Technology, Research School of Physics, Australian National University
- Henry N. Chapman
- Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY
- Adrian P. Mancuso
- European XFEL
- Anton Barty
- Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY
- Brian Abbey
- Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University
- Connie Darmanin
- Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University
- DOI
- https://doi.org/10.1038/s41467-022-32434-6
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 13
Abstract
Free-electron lasers are capable of high repetition rates and it is assumed that protein crystals often do not survive the first X-ray pulse. Here the authors address these issues with a demonstration of multi-hit serial crystallography in which multiple FEL pulses interact with the sample without destroying it.
