A sandwiched ventricular explant assay to model mouse coronary angiogenesis ex vivo

Abstract

Summary: Developing an ex vivo system that mimics in vivo developmental coronary angiogenesis provides an improved understanding of its underlying molecular and cellular mechanisms. Here, we present a sandwiched embryonic ventricular explant assay to model mouse coronary angiogenesis ex vivo. We describe steps for breeding mice, labeling endocardial cells, isolating murine hearts, dissecting left ventricles, and making sandwiched explants in Matrigel. We then detail procedures for modeling coronary angiogenesis and taking images.For complete details on the use and execution of this protocol, please refer to Lu et al. (2023)1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Cell Culture
• Developmental Biology
• Cell Differentiation