tANCHOR: a novel mammalian cell surface peptide display system
Daniel Ivanusic,
Kazimierz Madela,
Heidi Burghard,
Gudrun Holland,
Michael Laue,
Norbert Bannert
Affiliations
Daniel Ivanusic
1FG18 HIV & Other Retroviruses, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
Kazimierz Madela
2ZBS 4 Centre for Biological Threats & Special Pathogens: Advanced Light & Electron Microscopy, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
Heidi Burghard
1FG18 HIV & Other Retroviruses, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
Gudrun Holland
2ZBS 4 Centre for Biological Threats & Special Pathogens: Advanced Light & Electron Microscopy, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
Michael Laue
2ZBS 4 Centre for Biological Threats & Special Pathogens: Advanced Light & Electron Microscopy, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
Norbert Bannert
1FG18 HIV & Other Retroviruses, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
A novel tool for the presentation of peptides and small proteins on the surface of human cells has been developed. Our tANCHOR system utilizes tetraspanin anchors containing heterologous amino acid sequences inserted instead of the large extracellular loop. This technology allows a highly effective extracellular display of epitopes for antibody binding studies and many other potential applications.
