Micromachines
(Dec 2013)
Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry
Jing Yan,
Wolfgang-Andreas C. Bauer,
Martin Fischlechner,
Florian Hollfelder,
Clemens F. Kaminski,
Wilhelm T. S. Huck
Affiliations
Jing Yan
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
Wolfgang-Andreas C. Bauer
Melville Laboratory for Polymer Synthesis, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
Martin Fischlechner
Institute for Life Sciences, University of Southampton, Highfield Campus, Southampton SO17 1BJ, UK
Florian Hollfelder
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
Clemens F. Kaminski
Department of Chemical Engineering and Biotechnology, University of Cambridge, Pembroke Street, Cambridge CB2 3RA, UK
Wilhelm T. S. Huck
Melville Laboratory for Polymer Synthesis, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
DOI
https://doi.org/10.3390/mi4040402
Journal volume & issue
Vol. 4,
no. 4
Abstract
Read online
Here we report the application of monodisperse double emulsion droplets, produced in a single step within partially hydrophilic/partially hydrophobic microfluidic devices, as defined containers for quantitative flow cytometric analysis. Samples with varying fluorophore concentrations were generated, and a clear correlation between dye concentration and fluorescence signals was observed.
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