Strain-boosted hyperoxic graphene oxide efficiently loading and improving performances of microcystinase
Hong-Lin Liu,
Cai Cheng,
Ling-Zi Zuo,
Ming-Yue Yan,
Yan-Lin He,
Shi Huang,
Ming-Jing Ke,
Xiao-Liang Guo,
Yu Feng,
Hai-Feng Qian,
Ling-Ling Feng
Affiliations
Hong-Lin Liu
Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079 Hubei Province, China
Cai Cheng
Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079 Hubei Province, China
Ling-Zi Zuo
Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079 Hubei Province, China
Ming-Yue Yan
Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079 Hubei Province, China
Yan-Lin He
Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079 Hubei Province, China
Shi Huang
Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079 Hubei Province, China
Ming-Jing Ke
College of Environment, Zhejiang University of Technology, Hangzhou, 310014 Zhejiang Province, China
Xiao-Liang Guo
Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079 Hubei Province, China
Yu Feng
Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079 Hubei Province, China
Hai-Feng Qian
College of Environment, Zhejiang University of Technology, Hangzhou, 310014 Zhejiang Province, China; Corresponding author
Ling-Ling Feng
Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079 Hubei Province, China; Corresponding author
Summary: Harmful Microcystis blooms (HMBs) and microcystins (MCs) that are produced by Microcystis seriously threaten water ecosystems and human health. This study demonstrates an eco-friendly strategy for simultaneous removal of MCs and HMBs by adopting unique hyperoxic graphene oxides (HGOs) as carrier and pure microcystinase A (PMlrA) as connecting bridge to form stable HGOs@MlrA composite. After oxidation, HGOs yield inherent structural strain effects for boosting the immobilization of MlrA by material characterization and density functional theory calculations. HGO5 exhibits higher loading capacities for crude MlrA (1,559 mg·g−1) and pure MlrA (1,659 mg·g−1). Moreover, the performances of HGO5@MlrA composite, including the capability of removing MCs and HMBs, the ecological and human safety compared to MlrA or HGO5 treatment alone, have been studied. These results indicate that HGO5 can be used as a promising candidate material to effectively improve the application potential of MlrA in the simultaneous removal of MCs and HMBs.
