Purification of Phenolic Compounds from <i>Camellia polyodonta</i> Flower: Composition Analysis, Antioxidant Property, and Hypolipidemic Activity In Vitro and In Vivo
Zhuoya Xiang,
Li Liu,
Zhou Xu,
Qingbo Kong,
Heng Liang,
Shiling Feng,
Tao Chen,
Lijun Zhou,
Hongyu Yang,
Chunbang Ding
Affiliations
Zhuoya Xiang
College of Life Science, Sichuan Agricultural University, Ya’an 625014, China
Li Liu
College of Life Science, Sichuan Agricultural University, Ya’an 625014, China
Zhou Xu
Panxi Crops Research and Utilization Key Laboratory of Sichuan Province, Xichang University, Xichang 615000, China
Qingbo Kong
College of Life Science, Sichuan Agricultural University, Ya’an 625014, China
Heng Liang
College of Life Science, Sichuan Agricultural University, Ya’an 625014, China
Shiling Feng
College of Life Science, Sichuan Agricultural University, Ya’an 625014, China
Tao Chen
College of Life Science, Sichuan Agricultural University, Ya’an 625014, China
Lijun Zhou
College of Life Science, Sichuan Agricultural University, Ya’an 625014, China
Hongyu Yang
College of Life Science, Sichuan Agricultural University, Ya’an 625014, China
Chunbang Ding
College of Life Science, Sichuan Agricultural University, Ya’an 625014, China
Camellia polyodonta flowers are rich sources of phenolics and less attention has been paid to their potential biological activity. This study aims to explore the crude extracts and resulting purified fractions (CPFP-I, II, III, and IV) through compositional analysis and antioxidant and hypolipidemic activities in vitro and in vivo. Among four fractions, CPFP-II contained the highest total phenolic content and flavonoid content, while CPFP-III exhibited the greatest total proanthocyanidin content. Among the 14 phenolic compounds, CPFP-II displayed the highest content of procyanidin B2, B4, and C1, whereas CPFP-III contained the highest amount of 1,2,3,6-tetragalloylglucose. The DPPH, ABTS, and FRAP assessments demonstrated a consistent trend: CPFP-II > CPFP-III > CPFP-I > CPFP-IV. In vivo experiments showed that that all four fractions significantly reduced lipid levels in hyperlipidemic C. elegans (p C. polyodonta flower as a natural alternative.
