Memorias do Instituto Oswaldo Cruz (Feb 2008)

Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America times

  • Daniela Leles,
  • Adauto Araújo,
  • Luiz Fernando Ferreira,
  • Ana Carolina Paulo Vicente,
  • Alena Mayo Iñiguez

Journal volume & issue
Vol. 103, no. 1
pp. 106 – 108

Abstract

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Paleoparasitological studies using microscopy showed that Ascarisand Trichuris trichiura are the human intestinal parasites most found in archaeological sites. However, in pre-Columbian South American archaeological sites, Ascaris is rare. In this work we standardized a molecular methodology for Ascaris diagnosis directly from ancient DNA retrieved from coprolites. Using cythochrome b gene (142 bp) target, ancient DNA sequences were retrieved from South American samples, negative by microscopy. Moreover, the methodology applied was sensitive enough to detect ancient DNA extracted from 30 Ascaris eggs from an European coprolite. These results revealed a new scenery for the paleodistribution of Ascaris in South America.

Keywords