The Viromes of Mosquitoes from the Natural Landscapes of Western Siberia
Vladimir A. Ternovoi,
Alexander N. Shvalov,
Mikhail Yu. Kartashov,
Eugenia P. Ponomareva,
Natalia L. Tupota,
Yuri A. Khoroshavin,
Roman B. Bayandin,
Anastasia V. Gladysheva,
Tamara P. Mikryukova,
Tatyana V. Tregubchak,
Valery B. Loktev
Affiliations
Vladimir A. Ternovoi
State Research Center Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing of Russia, World-Class Genomic Research Center for Biological Safety and Technological Independence, 630559 Koltsovo, Russia
Alexander N. Shvalov
State Research Center Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing of Russia, World-Class Genomic Research Center for Biological Safety and Technological Independence, 630559 Koltsovo, Russia
Mikhail Yu. Kartashov
State Research Center Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing of Russia, World-Class Genomic Research Center for Biological Safety and Technological Independence, 630559 Koltsovo, Russia
Eugenia P. Ponomareva
State Research Center Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing of Russia, World-Class Genomic Research Center for Biological Safety and Technological Independence, 630559 Koltsovo, Russia
Natalia L. Tupota
State Research Center Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing of Russia, World-Class Genomic Research Center for Biological Safety and Technological Independence, 630559 Koltsovo, Russia
Yuri A. Khoroshavin
State Research Center Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing of Russia, World-Class Genomic Research Center for Biological Safety and Technological Independence, 630559 Koltsovo, Russia
Roman B. Bayandin
State Research Center Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing of Russia, World-Class Genomic Research Center for Biological Safety and Technological Independence, 630559 Koltsovo, Russia
Anastasia V. Gladysheva
State Research Center Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing of Russia, World-Class Genomic Research Center for Biological Safety and Technological Independence, 630559 Koltsovo, Russia
Tamara P. Mikryukova
State Research Center Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing of Russia, World-Class Genomic Research Center for Biological Safety and Technological Independence, 630559 Koltsovo, Russia
Tatyana V. Tregubchak
State Research Center Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing of Russia, World-Class Genomic Research Center for Biological Safety and Technological Independence, 630559 Koltsovo, Russia
Valery B. Loktev
State Research Center Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing of Russia, World-Class Genomic Research Center for Biological Safety and Technological Independence, 630559 Koltsovo, Russia
The metagenomic analysis of mosquitoes allows for the genetic characterization of mosquito-associated viruses in different regions of the world. This study applied a metagenomic approach to identify novel viral sequences in seven species of mosquitoes collected from the Novosibirsk region of western Siberia. Using NGS sequencing, we identified 15 coding-complete viral polyproteins (genomes) and 15 viral-like partial sequences in mosquitoes. The complete sequences for novel viruses or the partial sequences of capsid proteins, hypothetical viral proteins, and RdRps were used to identify their taxonomy. The novel viral sequences were classified within the orders Tymovirales and Picornavirales and the families Partitiviridae, Totiviridae, Tombusviridae, Iflaviridae, Nodaviridae, Permutotetraviridae, and Solemoviridae, with several attributed to four unclassified RNA viruses. Interestingly, the novel putative viruses and viral sequences were mainly associated with the mosquito Coquillettidia richardii. This study aimed to increase our understanding of the viral diversity in mosquitoes found in the natural habitats of Siberia, which is characterized by very long, snowy, and cold winters.
