Isolation, Identification and susceptibility of Candida species isolated from diabetic women referred to Kermanshah Diabetes Research Center (KDRC) in 2010.

Abstract

Background and Aim: Nearly 75% of all adult women have had at least one genital "yeast infection" in their lifetime. Patients with diabetes mellitus are risk for vulvo-vaginal candidiasis. The aim of this study is the determining species of Candida isolated from diabetic women referred to Kermanshah Diabetes Research Center (KDRC) in 2010. Materials and Methods: This descriptive – analytic study was performed on 100 diabetic women referred to Kermanshah Diabetes Research Center in 2010. All specimens were examined under direct microscopy and cultured on Sabouraud Dextrose Agar (SDA). Complimentary test such as germ tube test and Sugar Assimilation Test (API) were carried out to differentiate the Candida species. Performed Minimum Inhibitory Concentration (MIC) test for Candida species isolated. Statistical analysis was performed using the Chi-square test. All of the species isolate were sensitive to Amphotericin B, Fluconazole, and Clotrimazole. Results: From 100 samples that were under experience, 12% (12 cases) in direct microscopy test and 20% (20 cases) were infected to vaginal candidiasis by cultivation on Sabouraud Dextrose Agar (SDA). Frequency the Candida species isolated were: C.albicans with 62.5% (20 cases), C.glabrata with 18.7% (6 cases), C.tropicalis with 9.4% (3 cases) and C.parapsilosis with 9.4% (3 cases). Conclusions: C. albicans was, by far, the most predominant yeast isolate. Culture of vaginal discharge should be warranted because culture technique is more sensitive than direct smear.

Keywords

• Candidiasis
• diabetes mellitus
• C.albicans