Construction and validation of a multi-epitope in silico vaccine model for lymphatic filariasis by targeting Brugia malayi: a reverse vaccinology approach

Abstract

Highlights A novel computational approach was adopted for the development of a vaccine model against lymphatic filariasis. The potential epitopes were screened to test their antigenicity, toxicity, immunogenicity, and allergenicity. The prophylactic vaccine model was constructed with appropriate peptide linkers and the adjuvant—50 s ribosomal protein L7/L12. The tertiary structure of the vaccine model was validated with Ramachandran plot, ProSA, etc. Molecular docking against TLR4 receptor and immune simulation studies were performed to test the efficacy of the vaccine.

Keywords

• Immunoinformatics
• Lymphatic filariasis
• Brugia malayi
• Molecular docking
• Multi-epitope peptide-based vaccine
• Reverse vaccinology