Nature Communications (Dec 2022)
Biomolecular condensate phase diagrams with a combinatorial microdroplet platform
- William E. Arter,
- Runzhang Qi,
- Nadia A. Erkamp,
- Georg Krainer,
- Kieran Didi,
- Timothy J. Welsh,
- Julia Acker,
- Jonathan Nixon-Abell,
- Seema Qamar,
- Jordina Guillén-Boixet,
- Titus M. Franzmann,
- David Kuster,
- Anthony A. Hyman,
- Alexander Borodavka,
- Peter St George-Hyslop,
- Simon Alberti,
- Tuomas P. J. Knowles
Affiliations
- William E. Arter
- Yusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of Cambridge
- Runzhang Qi
- Yusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of Cambridge
- Nadia A. Erkamp
- Yusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of Cambridge
- Georg Krainer
- Yusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of Cambridge
- Kieran Didi
- Yusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of Cambridge
- Timothy J. Welsh
- Yusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of Cambridge
- Julia Acker
- Department of Biochemistry, University of Cambridge
- Jonathan Nixon-Abell
- Cambridge Institute for Medical Research, Department of Clinical Neurosciences, University of Cambridge
- Seema Qamar
- Cambridge Institute for Medical Research, Department of Clinical Neurosciences, University of Cambridge
- Jordina Guillén-Boixet
- Biotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität Dresden
- Titus M. Franzmann
- Biotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität Dresden
- David Kuster
- Max Planck Institute for Molecular Cell Biology and Genetics
- Anthony A. Hyman
- Max Planck Institute for Molecular Cell Biology and Genetics
- Alexander Borodavka
- Department of Biochemistry, University of Cambridge
- Peter St George-Hyslop
- Cambridge Institute for Medical Research, Department of Clinical Neurosciences, University of Cambridge
- Simon Alberti
- Biotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität Dresden
- Tuomas P. J. Knowles
- Yusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of Cambridge
- DOI
- https://doi.org/10.1038/s41467-022-35265-7
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 10
Abstract
A central concept for characterising phase-separating systems is the phase diagram but generation of such diagrams for biomolecular systems is typically slow and low-throughput. Here the authors describe PhaseScan, a combinatorial droplet microfluidic platform for high-resolution acquisition of multidimensional biomolecular phase diagrams.
