Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method

Fan Meng; Jiao Guo; Shan Wang; Changhui Zhang; Xuehui Li; Yanjuan Chen; Qiuli Shi; Liang Shao; Rundong Zhang; Ganggang Wang; Dan Su

Heliyon (Jun 2023)

Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method

Fan Meng,
Jiao Guo,
Shan Wang,
Changhui Zhang,
Xuehui Li,
Yanjuan Chen,
Qiuli Shi,
Liang Shao,
Rundong Zhang,
Ganggang Wang,
Dan Su

Affiliations

Fan Meng: Jujing-Chengdu Biotech, Chengdu, Sichuan, PR China
Jiao Guo: Jujing-Chengdu Biotech, Chengdu, Sichuan, PR China
Shan Wang: Jujing-Chengdu Biotech, Chengdu, Sichuan, PR China
Changhui Zhang: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China
Xuehui Li: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China
Yanjuan Chen: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China
Qiuli Shi: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China
Liang Shao: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China
Rundong Zhang: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China
Ganggang Wang: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, PR China; Corresponding author.
Dan Su: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China; Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, PR China; Corresponding author. State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China.

Journal volume & issue: Vol. 9, no. 6
p. e17364

Abstract

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The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a significant impact on global health. To address the urgent need for plasmids containing SARS-CoV-2 sequences in research, we have developed a high-throughput FastCloning platform for the construction of associated plasmids. Our platform uses a FastCloning method to construct a plasmid library from 29 ORFs of the virus and 20 commonly used vectors in the lab. The library contains 536 recombinant vectors, with a highly positive clone success rate of 92.4%. Our study provides a rapid and efficient approach to constructing a large plasmid library for SARS-CoV-2 research.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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