Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method
Fan Meng,
Jiao Guo,
Shan Wang,
Changhui Zhang,
Xuehui Li,
Yanjuan Chen,
Qiuli Shi,
Liang Shao,
Rundong Zhang,
Ganggang Wang,
Dan Su
Affiliations
Fan Meng
Jujing-Chengdu Biotech, Chengdu, Sichuan, PR China
Jiao Guo
Jujing-Chengdu Biotech, Chengdu, Sichuan, PR China
Shan Wang
Jujing-Chengdu Biotech, Chengdu, Sichuan, PR China
Changhui Zhang
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China
Xuehui Li
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China
Yanjuan Chen
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China
Qiuli Shi
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China
Liang Shao
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China
Rundong Zhang
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China
Ganggang Wang
Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, PR China; Corresponding author.
Dan Su
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China; Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, PR China; Corresponding author. State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China.
The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a significant impact on global health. To address the urgent need for plasmids containing SARS-CoV-2 sequences in research, we have developed a high-throughput FastCloning platform for the construction of associated plasmids. Our platform uses a FastCloning method to construct a plasmid library from 29 ORFs of the virus and 20 commonly used vectors in the lab. The library contains 536 recombinant vectors, with a highly positive clone success rate of 92.4%. Our study provides a rapid and efficient approach to constructing a large plasmid library for SARS-CoV-2 research.
