Leaf Morphology Genes SRL1 and RENL1 Co-Regulate Cellulose Synthesis and Affect Rice Drought Tolerance
Liu Dan,
Zhao Huibo,
Wang Zi’an,
Xu Jing,
Liu Yiting,
Wang Jiajia,
Chen Minmin,
Liu Xiong,
Zhang Zhihai,
Cen Jiangsu,
Zhu Li,
Hu Jiang,
Ren Deyong,
Gao Zhenyu,
Dong Guojun,
Zhang Qiang,
Shen Lan,
Li Qing,
Qian Qian,
Hu Songping,
Zhang Guangheng
Affiliations
Liu Dan
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Research Center of Plant Functional Genes and Tissue Culture Technology, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China
Zhao Huibo
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Qian Qian Academician Workstation, National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China
Wang Zi’an
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Research Center of Plant Functional Genes and Tissue Culture Technology, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China
Xu Jing
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Liu Yiting
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Research Center of Plant Functional Genes and Tissue Culture Technology, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China
Wang Jiajia
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Chen Minmin
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Liu Xiong
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Zhang Zhihai
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Cen Jiangsu
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Zhu Li
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Qian Qian Academician Workstation, National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China
Hu Jiang
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Ren Deyong
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Gao Zhenyu
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Dong Guojun
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Zhang Qiang
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Shen Lan
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Li Qing
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Qian Qian
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Qian Qian Academician Workstation, National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China; Corresponding authors:
Hu Songping
Research Center of Plant Functional Genes and Tissue Culture Technology, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China; Corresponding authors: (
Zhang Guangheng
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Qian Qian Academician Workstation, National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China; Corresponding authors:
The morphological development of rice (Oryza sativa L.) leaves is closely related to plant architecture, physiological activities, and resistance. However, it is unclear whether there is a co-regulatory relationship between the morphological development of leaves and adaptation to drought environment. In this study, a drought-sensitive, roll-enhanced, and narrow-leaf mutant (renl1) was induced from a semi-rolled leaf mutant (srl1) by ethyl methane sulfonate (EMS), which was obtained from Nipponbare (NPB) through EMS. Map-based cloning and functional validation showed that RENL1 encodes a cellulose synthase, allelic to NRL1/OsCLSD4. The RENL1 mutation resulted in reduced vascular bundles, vesicular cells, cellulose, and hemicellulose contents in cell walls, diminishing the water-holding capacity of leaves. In addition, the root system of the renl1 mutant was poorly developed and its ability to scavenge reactive oxygen species (ROS) was decreased, leading to an increase in ROS after drought stress. Meanwhile, genetic results showed that RENL1 and SRL1 synergistically regulated cell wall components. Our results revealed a theoretical basis for further elucidating the molecular regulation mechanism of cellulose on rice drought tolerance, and provided a new genetic resource for enhancing the synergistic regulation network of plant type and stress resistance, thereby realizing simultaneous improvement of multiple traits in rice.
