Leaf Morphology Genes SRL1 and RENL1 Co-Regulate Cellulose Synthesis and Affect Rice Drought Tolerance

Liu Dan; Zhao Huibo; Wang Zi’an; Xu Jing; Liu Yiting; Wang Jiajia; Chen Minmin; Liu Xiong; Zhang Zhihai; Cen Jiangsu; Zhu Li; Hu Jiang; Ren Deyong; Gao Zhenyu; Dong Guojun; Zhang Qiang; Shen Lan; Li Qing; Qian Qian; Hu Songping; Zhang Guangheng

Rice Science (Jan 2024)

Leaf Morphology Genes SRL1 and RENL1 Co-Regulate Cellulose Synthesis and Affect Rice Drought Tolerance

Liu Dan,
Zhao Huibo,
Wang Zi’an,
Xu Jing,
Liu Yiting,
Wang Jiajia,
Chen Minmin,
Liu Xiong,
Zhang Zhihai,
Cen Jiangsu,
Zhu Li,
Hu Jiang,
Ren Deyong,
Gao Zhenyu,
Dong Guojun,
Zhang Qiang,
Shen Lan,
Li Qing,
Qian Qian,
Hu Songping,
Zhang Guangheng

Affiliations

Liu Dan: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Research Center of Plant Functional Genes and Tissue Culture Technology, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China
Zhao Huibo: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Qian Qian Academician Workstation, National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China
Wang Zi’an: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Research Center of Plant Functional Genes and Tissue Culture Technology, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China
Xu Jing: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Liu Yiting: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Research Center of Plant Functional Genes and Tissue Culture Technology, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China
Wang Jiajia: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Chen Minmin: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Liu Xiong: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Zhang Zhihai: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Cen Jiangsu: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Zhu Li: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Qian Qian Academician Workstation, National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China
Hu Jiang: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Ren Deyong: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Gao Zhenyu: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Dong Guojun: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Zhang Qiang: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Shen Lan: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Li Qing: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Qian Qian: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Qian Qian Academician Workstation, National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China; Corresponding authors:
Hu Songping: Research Center of Plant Functional Genes and Tissue Culture Technology, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China; Corresponding authors: (
Zhang Guangheng: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Qian Qian Academician Workstation, National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China; Corresponding authors:

Journal volume & issue: Vol. 31, no. 1
pp. 103 – 117

Abstract

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The morphological development of rice (Oryza sativa L.) leaves is closely related to plant architecture, physiological activities, and resistance. However, it is unclear whether there is a co-regulatory relationship between the morphological development of leaves and adaptation to drought environment. In this study, a drought-sensitive, roll-enhanced, and narrow-leaf mutant (renl1) was induced from a semi-rolled leaf mutant (srl1) by ethyl methane sulfonate (EMS), which was obtained from Nipponbare (NPB) through EMS. Map-based cloning and functional validation showed that RENL1 encodes a cellulose synthase, allelic to NRL1/OsCLSD4. The RENL1 mutation resulted in reduced vascular bundles, vesicular cells, cellulose, and hemicellulose contents in cell walls, diminishing the water-holding capacity of leaves. In addition, the root system of the renl1 mutant was poorly developed and its ability to scavenge reactive oxygen species (ROS) was decreased, leading to an increase in ROS after drought stress. Meanwhile, genetic results showed that RENL1 and SRL1 synergistically regulated cell wall components. Our results revealed a theoretical basis for further elucidating the molecular regulation mechanism of cellulose on rice drought tolerance, and provided a new genetic resource for enhancing the synergistic regulation network of plant type and stress resistance, thereby realizing simultaneous improvement of multiple traits in rice.

Published in Rice Science

ISSN: 1672-6308 (Print); 1876-4762 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Plant culture
Website: https://www.journals.elsevier.com/rice-science

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