A 3-D printed vitrification device integrated with French straws
Nolan J. Tiersch,
Jacqueline Paulsen,
Yue Liu,
Terrence R. Tiersch
Affiliations
Nolan J. Tiersch
Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, LA 70803, USA
Jacqueline Paulsen
Department of Biological and Agricultural Engineering, Louisiana State University and Agricultural Center, Baton Rouge, LA 70803, USA
Yue Liu
Department of Biological and Agricultural Engineering, Louisiana State University and Agricultural Center, Baton Rouge, LA 70803, USA; Aquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, LA 70820, USA; Corresponding author at: Aquatic Germplasm and Genetic Resources Center, Louisiana State University Agricultural Center, Baton Rouge, LA, 70820.
Terrence R. Tiersch
Aquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, LA 70820, USA
The goal of this work was to develop prototypes of open-hardware vitrification devices for sperm cryopreservation that can be integrated with existing straw platforms. The open-hardware Vitrification Device for French Straws (VD-FS) is low-cost, customizable, 3-D printable, standardized, and allows long-term sample storage and identification. The feasibility was shown for vitrifying and storing samples with multiple configurations. The results can be improved by design alternation and evaluation of various vitrification solutions. This is the first complete open-hardware vitrification device that can be integrated with existing French-straw storage systems, providing a foundation for future community-level modifications and improvements.
