Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (<i>Zea mays</i>) Seedlings under Drought Stress
Phamela Acosta-Pérez,
Bianka Dianey Camacho-Zamora,
Edward A. Espinoza-Sánchez,
Guadalupe Gutiérrez-Soto,
Francisco Zavala-García,
María Jazmín Abraham-Juárez,
Sugey Ramona Sinagawa-García
Affiliations
Phamela Acosta-Pérez
Universidad Autónoma de Nuevo León, Facultad de Agronomía, Lab. de Biotecnología, Calle Francisco I. Madero S/N, Hacienda el Canadá, Cd Gral. Escobedo 66050, N.L., Mexico
Bianka Dianey Camacho-Zamora
Unidad de Genómica, Centro de Investigación y Desarrollo en Ciencias de la Salud, Campus Ciencias de la Salud, UANL, Dr. Carlos Canseco s/n, Mitras Centro, Monterrey 64460, N.L, Mexico
Edward A. Espinoza-Sánchez
Universidad Autónoma de Chihuahua, Facultad de Ciencias Químicas, Circuito Universitario S/N, Campus Uach II, Chihuahua 31125, Chih, Mexico
Guadalupe Gutiérrez-Soto
Universidad Autónoma de Nuevo León, Facultad de Agronomía, Lab. de Biotecnología, Calle Francisco I. Madero S/N, Hacienda el Canadá, Cd Gral. Escobedo 66050, N.L., Mexico
Francisco Zavala-García
Universidad Autónoma de Nuevo León, Facultad de Agronomía, Lab. de Biotecnología, Calle Francisco I. Madero S/N, Hacienda el Canadá, Cd Gral. Escobedo 66050, N.L., Mexico
María Jazmín Abraham-Juárez
División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José, Lomas 4 sección, San Luis Potosí 78216, S.L.P., Mexico
Sugey Ramona Sinagawa-García
Universidad Autónoma de Nuevo León, Facultad de Agronomía, Lab. de Biotecnología, Calle Francisco I. Madero S/N, Hacienda el Canadá, Cd Gral. Escobedo 66050, N.L., Mexico
Maize is the most important crop around the world and it is highly sensitive to abiotic stress caused by drought, excessive salinity, and extreme temperature. In plants, trehalose has been widely studied for its role in plant adaptation to different abiotic stresses such as drought, high and low temperature, and osmotic stress. Thus, the aim of this work was to clone and characterize at molecular level the trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) genes from maize and to evaluate its differential expression in maize seedlings under drought stress. To carry out this, resistant and susceptible maize lines were subjected to drought stress during 72 h. Two full-length cDNAs of TPS and one of TPP were cloned and sequenced. Then, TPS and TPP amino acid sequences were aligned with their homologs from different species, showing highly conserved domains and the same catalytic sites. Relative expression of both genes was evaluated by RT-qPCR at different time points. The expression pattern showed significant induction after 0.5 h in resistant lines and after two to four hours in susceptible plants, showing their participation in drought stress response.
